Crystal structure of yeast mitochondrial peripheral membrane protein Tim44p C-terminal domain.

Journal of Molecular Biology
Ratnakar JosyulaBingdong Sha

Abstract

The protein transports from the cell cytosol to the mitochondria matrix are carried out by the translocase of the outer membrane (TOM) complex and the translocase of the inner membrane (TIM) complexes. Tim44p is an essential mitochondrial peripheral membrane protein and a major component of TIM23 translocon. Tim44p can tightly associate with the inner mitochondrial membrane. To investigate the mechanism by which Tim44p functions in the TIM23 translocon to deliver the mitochondrial protein precursors, we have determined the crystal structure of the yeast Tim44p C-terminal domain to 3.2A resolution using the MAD method. The Tim44p C-terminal domain forms a monomer in the crystal structure and contains six alpha-helices and four antiparallel beta-strands. A large hydrophobic pocket was identified on the Tim44p structure surface. The N-terminal helix A1 is positively charged and the helix A1 protrudes out from the Tim44p main body.

References

Jan 1, 1997·Annual Review of Biochemistry·W Neupert
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Mar 5, 1999·Science·M W GrayB F Lang
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·T C Terwilliger, J Berendzen
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·C WeissA Azem
Jun 6, 2000·Current Biology : CB·N Pfanner
Aug 16, 2000·Acta Crystallographica. Section D, Biological Crystallography·T C Terwilliger
Dec 26, 2001·The Journal of Biological Chemistry·Fernando MoroMichael Brunner
Aug 3, 2002·Nature Reviews. Molecular Cell Biology·Walter Neupert, Michael Brunner
Apr 5, 2003·Science·Qinglian LiuElizabeth A Craig
Oct 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Albert SickmannChris Meisinger
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Carla M Koehler
Oct 19, 2004·Nature Structural & Molecular Biology·Patrick D'SilvaElizabeth A Craig
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Ratnakar JosyulaBingdong Sha

❮ Previous
Next ❯

Citations

Feb 1, 2007·Annual Review of Biochemistry·Walter Neupert, Johannes M Herrmann
Feb 14, 2015·Journal of Molecular Biology·Walter Neupert
Sep 11, 2007·Trends in Cell Biology·Michael J BakerMichael T Ryan
Dec 18, 2014·Protein Science : a Publication of the Protein Society·Susanne E HorvathNikolaus Pfanner
Jul 27, 2010·Biochimica Et Biophysica Acta·Toshiya EndoShin Kawano
Feb 2, 2010·Biochimica Et Biophysica Acta·Dejana Mokranjac, Walter Neupert
Dec 30, 2014·Trends in Cell Biology·Christian SchulzPeter Rehling
Aug 2, 2008·Biochimica Et Biophysica Acta·Dejana Mokranjac, Walter Neupert
Mar 30, 2016·Annual Review of Biochemistry·Basil J Greber, Nenad Ban
Sep 16, 2016·Cell and Tissue Research·Phanee ManganasKostas Tokatlidis
Jun 10, 2015·Molecular and Cellular Biology·Eva MartincováPavel Doležal
Jan 19, 2018·BMC Biology·Elizabeth A Craig
Feb 9, 2017·The Journal of Biological Chemistry·Non MiyataCarla M Koehler
Sep 21, 2007·The Journal of Biological Chemistry·Olga Slutsky-LeidermanAbdussalam Azem
Oct 1, 2021·Nature Communications·Ridhima GomkalePeter Rehling

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Acta Crystallographica. Section D, Biological Crystallography
Noriko HandaShigeyuki Yokoyama
The Journal of Biological Chemistry
Dejana MokranjacWalter Neupert
Proceedings of the National Academy of Sciences of the United States of America
Patrick D'SilvaElizabeth A Craig
© 2022 Meta ULC. All rights reserved