Crystal structure of YjeQ from Thermotoga maritima contains a circularly permuted GTPase domain

Proceedings of the National Academy of Sciences of the United States of America
Dong Hae ShinSung-Hou Kim

Abstract

We have determined the crystal structure of the GDP complex of the YjeQ protein from Thermotoga maritima (TmYjeQ), a member of the YjeQ GTPase subfamaily. TmYjeQ, a homologue of Escherichia coli YjeQ, which is known to bind to the ribosome, is composed of three domains: an N-terminal oligonucleotide/oligosaccharide-binding fold domain, a central GTPase domain, and a C-terminal zinc-finger domain. The crystal structure of TmYjeQ reveals two interesting domains: a circularly permutated GTPase domain and an unusual zinc-finger domain. The binding mode of GDP in the GTPase domain of TmYjeQ is similar to those of GDP or GTP analogs in ras proteins, a prototype GTPase. The N-terminal oligonucleotide/oligosaccharide-binding fold domain, together with the GTPase domain, forms the extended RNA-binding site. The C-terminal domain has an unusual zinc-finger motif composed of Cys-250, Cys-255, Cys-263, and His-257, with a remote structural similarity to a portion of a DNA-repair protein, rad51 fragment. The overall structural features of TmYjeQ make it a good candidate for an RNA-binding protein, which is consistent with the biochemical data of the YjeQ subfamily in binding to the ribosome.

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Citations

Feb 18, 2006·Molecular Genetics and Genomics : MGG·Lionel CladièreSimone J Séror
Sep 4, 2007·Journal of Structural and Functional Genomics·Dong Hae ShinSung-Hou Kim
Jul 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Qiang GuoNing Gao
Sep 20, 2008·The Journal of Biological Chemistry·Magali MoreauDaniel F Klessig
Mar 20, 2013·Journal of Biochemistry·Simon GotoHyouta Himeno
Nov 17, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·C E NicholsD K Stammers
Jul 25, 2006·Infection and Immunity·Tracey L CampbellEric D Brown
Dec 8, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Eric D Brown
Aug 19, 2011·BMC Bioinformatics·Paweł Daniluk, Bogdan Lesyng
Nov 17, 2007·BMC Structural Biology·Alexej Abyzov, Valentin A Ilyin
Jun 17, 2011·Annual Review of Biochemistry·Alfred Wittinghofer, Ingrid R Vetter
Aug 30, 2008·Journal of Molecular Recognition : JMR·Chia-Han ChuTun-Wen Pai
Nov 26, 2010·The EMBO Journal·Simon GotoHyouta Himeno
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Jul 1, 2008·Journal of Molecular Biology·Takatsugu KimuraHyouta Himeno
May 10, 2017·Nucleic Acids Research·Jorge Pedro López-AlonsoSean R Connell
Jun 15, 2007·Critical Reviews in Biochemistry and Molecular Biology·Daniel N Wilson, Knud H Nierhaus
Apr 12, 2017·Proceedings of the National Academy of Sciences of the United States of America·Aida RaziJoaquin Ortega
Apr 15, 2005·The Biochemical Journal·Tracey L CampbellEric D Brown

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