Crystal structures of γ-glutamylmethylamide synthetase provide insight into bacterial metabolism of oceanic monomethylamine.

The Journal of Biological Chemistry
Ning WangChun-Yang Li

Abstract

Monomethylamine (MMA) is an important climate-active oceanic trace gas and ubiquitous in the oceans. γ-Glutamylmethylamide synthetase (GmaS) catalyzes the conversion of MMA to γ-glutamylmethylamide, the first step in MMA metabolism in many marine bacteria. The gmaS gene occurs in ∼23% of microbial genomes in the surface ocean and is a validated biomarker to detect MMA-utilizing bacteria. However, the catalytic mechanism of GmaS has not been studied because of the lack of structural information. Here, the GmaS from Rhodovulum sp. 12E13 (RhGmaS) was characterized, and the crystal structures of apo-RhGmaS and RhGmaS with different ligands in five states were solved. Based on structural and biochemical analyses, the catalytic mechanism of RhGmaS was explained. ATP is first bound in RhGmaS, leading to a conformational change of a flexible loop (Lys287-Ile305), which is essential for the subsequent binding of glutamate. During the catalysis of RhGmaS, the residue Arg312 participates in polarizing the γ-phosphate of ATP and in stabilizing the γ-glutamyl phosphate intermediate; Asp177 is responsible for the deprotonation of MMA, assisting the attack of MMA on γ-glutamyl phosphate to produce a tetrahedral intermediate; and Glu186 acts a...Continue Reading

References

Apr 1, 1987·Journal of Bacteriology·M Husain, V L Davidson
Jun 1, 1994·Journal of Molecular Evolution·J R BrownW F Doolittle
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y KumadaY Tateno
Nov 1, 1995·Protein Science : a Publication of the Protein Society·S H LiawD Eisenberg
Mar 10, 2000·Biochimica Et Biophysica Acta·D EisenbergS H Rotstein
Jan 30, 2004·Nature·Natascha SeljeThorsten Brinkhoff
Jul 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Wojciech W KrajewskiSherry L Mowbray
Jul 11, 2006·The Journal of Biological Chemistry·Hideaki UnnoMasami Kusunoki
Jul 21, 2006·Acta Crystallographica. Section D, Biological Crystallography·Wladek MinorMaksymilian Chruszcz
Feb 8, 2007·Bioscience, Biotechnology, and Biochemistry·Sachiko YamamotoTakashi Tachiki
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Apr 20, 2010·Applied and Environmental Microbiology·Yin ChenJ Colin Murrell
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Oct 19, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yin ChenJ Colin Murrell
Jul 24, 2012·Chemical Society Reviews·Lucy J CarpenterRachael Beale
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Mar 8, 2014·The Science of the Total Environment·Amanda E PosteRichard F Wright
Jan 1, 1992·Bioscience, Biotechnology, and Biochemistry·T KimuraY Tonomura
Mar 1, 2017·Environmental Microbiology·Martin TaubertJ Colin Murrell
Mar 4, 2017·Environmental Microbiology·Lisa Y Stein

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