Crystal structures of a type-1 ribosome inactivating protein from Momordica balsamina in the bound and unbound states

Biochimica Et Biophysica Acta
Gajraj Singh KushwahaTej P Singh

Abstract

The ribosome inactivating proteins (RIPs) of type 1 are plant toxins that eliminate adenine base selectively from the single stranded loop of rRNA. We report six crystal structures, type 1 RIP from Momordica balsamina (A), three in complexed states with ribose (B), guanine (C) and adenine (D) and two structures of MbRIP-1 when crystallized with adenosine triphosphate (ATP) (E) and 2'-deoxyadenosine triphosphate (2'-dATP) (F). These were determined at 1.67Å, 1.60Å, 2.20Å, 1.70Å, 2.07Å and 1.90Å resolutions respectively. The structures contained, (A) unbound protein molecule, (B) one protein molecule and one ribose sugar, (C) one protein molecule and one guanine base, (D) one protein molecule and one adenine base, (E) one protein molecule and one ATP-product adenine molecule and (F) one protein molecule and one 2'-dATP-product adenine molecule. Three distinct conformations of the side chain of Tyr70 were observed with (i) χ(1)=-66°and χ(2)=165° in structures (A) and (B); (ii) χ(1)=-95° and χ(2)=70° in structures (C), (D) and (E); and (iii) χ(1)=-163° and χ(2)=87° in structure (F). The conformation of Tyr70 in (F) corresponds to the structure of a conformational intermediate. This is the first structure which demonstrates that the...Continue Reading

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Citations

May 27, 2015·Toxins·Joachim SchrotMatthias F Melzig
Nov 8, 2017·The FEBS Journal·Alexander WlodawerBernhard Rupp
Sep 2, 2015·Acta Crystallographica. Section F, Structural Biology Communications·M VinkovicR Gill
Jul 13, 2020·International Journal of Biological Macromolecules·Xiaojiao FanTengchuan Jin
Dec 10, 2016·The Journal of Physical Chemistry. B·Stefan A P LenzStacey D Wetmore

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