Crystal structures of artocarpin, a Moraceae lectin with mannose specificity, and its complex with methyl-alpha-D-mannose: implications to the generation of carbohydrate specificity

Journal of Molecular Biology
J V PratapM Vijayan

Abstract

The seeds of jack fruit (Artocarpus integrifolia) contain two tetrameric lectins, jacalin and artocarpin. Jacalin was the first lectin found to exhibit the beta-prism I fold, which is characteristic of the Moraceae plant lectin family. Jacalin contains two polypeptide chains produced by a post-translational proteolysis which has been shown to be crucial for generating its specificity for galactose. Artocarpin is a single chain protein with considerable sequence similarity with jacalin. It, however, exhibits many properties different from those of jacalin. In particular, it is specific to mannose. The structures of two crystal forms, form I and form II, of the native lectin have been determined at 2.4 and 2.5 A resolution, respectively. The structure of the lectin complexed with methyl-alpha-mannose, has also been determined at 2.9 A resolution. The structure is similar to jacalin, although differences exist in details. The crystal structures and detailed modelling studies indicate that the following differences between the carbohydrate binding sites of artocarpin and jacalin are responsible for the difference in the specificities of the two lectins. Firstly, artocarpin does not contain, unlike jacalin, an N terminus generated b...Continue Reading

References

Feb 1, 1979·International Journal of Peptide and Protein Research·T N BhatM Vijayan
Jul 5, 1991·Journal of Immunological Methods·I K de Miranda-SantosA Campos-Neto
Oct 13, 1989·Science·N Sharon, H Lis
Oct 5, 1989·Journal of Molecular Biology·C S Wright
Feb 14, 1971·Journal of Molecular Biology·B Lee, F M Richards
Jan 1, 1981·Advances in Protein Chemistry·J S Richardson
Dec 20, 1993·Journal of Molecular Biology·M C Lawrence, P M Colman
Jul 1, 1996·Nature Structural Biology·R SankaranarayananM Vijayan
Jan 1, 1996·Annual Review of Biochemistry·W I Weis, K Drickamer
Apr 1, 1997·Journal of Molecular Graphics & Modelling·R M Esnouf
Apr 18, 1998·Biochimica Et Biophysica Acta·R LorisL Wyns
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·S C LovellD C Richardson
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·C SadasivanM Vijayan
Oct 6, 1999·Current Opinion in Structural Biology·K Drickamer
Oct 6, 1999·Current Opinion in Structural Biology·J M Rini, Y D Lobsanov
Dec 23, 1999·Current Opinion in Structural Biology·M Vijayan, N Chandra
Feb 19, 2002·Chemical Reviews·Halina Lis, Nathan Sharon
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4

❮ Previous
Next ❯

Citations

Jan 10, 2013·Glycoconjugate Journal·Maria A SouzaMaria Cristina Roque-Barreira
Jul 18, 2006·Nucleic Acids Research·K SumathiK Sekar
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Francisca Gallego del SolJuan J Calvete
May 7, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Mads GabrielsenRichard J Cogdell
May 6, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Ashok K PatelPetri Kursula
Jun 8, 2007·Microbiology and Molecular Biology Reviews : MMBR·Craig R Pigott, David J Ellar
Sep 9, 2006·Bioscience, Biotechnology, and Biochemistry·Tomokazu HaraguchiFumio Yagi
Aug 27, 2014·Bioscience, Biotechnology, and Biochemistry·Michiko ShimokawaJun Hirabayashi
Jul 6, 2016·BMC Immunology·Valéria Cintra Barbosa-LorenziConstance Oliver
Jul 1, 2017·International Journal of Molecular Sciences·Thiago Aparecido da SilvaMaria Cristina Roque-Barreira
Jul 15, 2016·International Journal of Biological Macromolecules·Alfa Umaro BariBenildo Sousa Cavada
Jun 26, 2003·Protein Science : a Publication of the Protein Society·Dong Hae ShinSung-Hou Kim
Oct 23, 2015·Proteins·K V AbhinavM Vijayan
Dec 20, 2018·The Biochemical Journal·Antonio Eufrásio Vieira NetoRenato de Azevedo Moreira
Jul 4, 2006·Protein Science : a Publication of the Protein Society·Krishnamurthy N RaoSubramanyam Swaminathan
Mar 10, 2005·Journal of Biomolecular NMR·Nozomi SugimoriMasatsune Kainosho
Feb 9, 2008·Proteins·Garima GuptaAvadhesha Surolia
May 17, 2008·Chemistry : a European Journal·Sophie VandenbusscheKristin Bartik
Mar 10, 2006·Organic & Biomolecular Chemistry·Sneha Sudha KomathMusti J Swamy
Jul 31, 2013·Acta Crystallographica. Section D, Biological Crystallography·Alok SharmaM Vijayan
Jun 10, 2005·Glycobiology·Jennifer L MeagherJeanne A Stuckey
May 3, 2014·The Journal of Biological Chemistry·Mayumi KanagawaYoshiki Yamaguchi
Jul 8, 2017·Bioscience Reports·Felipe Domingos de SousaRenato de Azevedo Moreira
Jan 13, 2019·International Journal of Molecular Sciences·Annick BarrePierre Rougé
Jun 11, 2014·Acta Crystallographica. Section F, Structural Biology Communications·Mads GabrielsenRichard J Cogdell
Feb 11, 2015·Acta Crystallographica. Section D, Biological Crystallography·K V AbhinavM Vijayan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.