Crystal structures of Bbp from Staphylococcus aureus reveal the ligand binding mechanism with Fibrinogen α

Protein & Cell
Xinyue ZhangMaojun Yang

Abstract

Bone sialoprotein-binding protein (Bbp), a MSCRAMMs (Microbial Surface Components Recognizing Adhesive Matrix Molecules) family protein expressed on the surface of Staphylococcus aureus (S. aureus), mediates adherence to fibrinogen α (Fg α), a component in the extracellular matrix of the host cell and is important for infection and pathogenesis. In this study, we solved the crystal structures of apo-Bbp(273-598) and Bbp(273-598)-Fg α(561-575) complex at a resolution of 2.03 Å and 1.45 Å, respectively. Apo-Bbp(273-598) contained the ligand binding region N2 and N3 domains, both of which followed a DE variant IgG fold characterized by an additional D1 strand in N2 domain and D1' and D2' strands in N3 domain. The peptide mapped to the Fg α(561-575) bond to Bbp(273-598) on the open groove between the N2 and N3 domains. Strikingly, the disordered C-terminus in the apo-form reorganized into a highly-ordered loop and a β-strand G'' covering the ligand upon ligand binding. Bbp(Ala298-Gly301) in the N2 domain of the Bbp(273-598)-Fg α(561-575) complex, which is a loop in the apo-form, formed a short α-helix to interact tightly with the peptide. In addition, Bbp(Ser547-Gln561) in the N3 domain moved toward the binding groove to make conta...Continue Reading

References

Jan 1, 1994·Annual Review of Microbiology·J M PattiM Höök
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G K Hunter, H A Goldberg
Jun 25, 1998·Infection and Immunity·A F GillaspyM S Smeltzer
Aug 26, 1998·The New England Journal of Medicine·F D Lowy
Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·B GanssJ Sodek
Jul 20, 2001·Annals of the New York Academy of Sciences·M W MosessonD A Meh
Aug 17, 2002·Infection and Immunity·Sharon J PeacockNicholas P J Day
Oct 24, 2002·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsThomas C Terwilliger
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Apr 28, 2005·The Lancet Infectious Diseases·Nicola ZetolaWilliam R Bishai
Sep 13, 2007·Thrombosis and Haemostasis·Jose RiveraPietro Speziale
Mar 20, 2009·Biochemistry·Justin M KollmanRussell F Doolittle
Oct 12, 2010·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Stan Deresinski, Victor Herrera
May 6, 2011·The Journal of Biological Chemistry·Vannakambadi K GaneshMagnus Höök
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

❮ Previous
Next ❯

Citations

Oct 25, 2017·Current Allergy and Asthma Reports·Tammy GonzalezGurjit K Khurana Hershey
Jul 12, 2019·Journal of Cheminformatics·Lee SteinbergJeremy Frey
Dec 3, 2016·Protein & Cell·Hao Cheng
Mar 31, 2018·Science·Lukas F MillesRafael C Bernardi
Aug 15, 2020·Microbiology and Molecular Biology Reviews : MMBR·Katrin Schilcher, Alexander R Horswill
May 12, 2017·The Biochemical Journal·Andrew B Herr, Alexander W Thorman
Jun 23, 2021·MBio·Hyun Jung JiPaul M Sullam
Aug 25, 2021·Seminars in Thrombosis and Hemostasis·Katherine J KearneyFraser L Macrae

❮ Previous
Next ❯

Methods Mentioned

BETA
surface plasmon resonance
chip
PCR
gel filtration

Software Mentioned

PyMOL
CCP4
COOT
Origin
HKL2000
PHENIX

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.