Crystal structures of bovine odorant-binding protein in complex with odorant molecules

European Journal of Biochemistry
Florence VincentC Cambillau

Abstract

The structure of bovine odorant-binding protein (bOBP) revealed a striking feature of a dimer formed by domain swapping [Tegoni, M., Ramoni, R., Bignetti, E., Spinelli, S. & Cambillau, C. (1996) Nat. Struct. Biol.3, 863-867; Bianchet, M.A., Bains, G., Pelosi, P., Pevsner, J., Snyder, S.H., Monaco, H.L. & Amzel, L.M. (1996) Nat. Struct. Biol.3, 934-939] and the presence of a naturally occuring ligand [Ramoni, R., Vincent, F., Grolli, S., Conti, V., Malosse, C., Boyer, F.D., Nagnan-Le Meillour, P., Spinelli, S., Cambillau, C. & Tegoni, M. (2001) J. Biol. Chem.276, 7150-7155]. These features led us to investigate the binding of odorant molecules with bOBP in solution and in the crystal. The behavior of odorant molecules in bOBP resembles that observed with porcine OBP (pOBP), although the latter is monomeric and devoid of ligand when purified. The odorant molecules presented K(d) values with bOBP in the micromolar range. Most of the X-ray structures revealed that odorant molecules interact with a common set of residues forming the cavity wall and do not exhibit specific interactions. Depending on the ligand and on the monomer (A or B), a single residue--Phe89--presents alternate conformations and might control cross-talking betwee...Continue Reading

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Citations

Oct 30, 2012·Analytical and Bioanalytical Chemistry·Federica BianchiRoberto Ramoni
Apr 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Christopher A ButtsIvan J Dmochowski
Jul 7, 2011·International Journal of Molecular Sciences·Eugenia PolveriniRoberto Favilla
Feb 14, 2008·Biochimica Et Biophysica Acta·Roberto RamoniMariella Tegoni
Jul 3, 2007·Veterinary Immunology and Immunopathology·Gordon B MitchellJeff L Caswell
Feb 8, 2007·Proteins·Jérôme GolebiowskiDaniel Cabrol-Bass
Jan 22, 2013·Journal of Molecular Recognition : JMR·Gabriel Gutiérrez-MagdalenoEnrique García-Hernández
Mar 1, 2006·Journal of Plant Physiology·Joanna GrzybKazimierz Strzałka
Mar 27, 2015·Proteins·André SchiefnerArne Skerra
Apr 13, 2007·Journal of Structural Biology·Alberto MazziniRoberto Favilla
Jul 28, 2016·International Journal of Biological Macromolecules·Martiniano BelloJosé Correa Basurto
Sep 10, 2010·Veterinary Pathology·G B MitchellJ L Caswell
Sep 6, 2018·PloS One·Alessandro CapoMaria Staiano
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Jun 21, 2018·Scientific Reports·Subramanian MuthukumarGovindaraju Archunan
Dec 17, 2019·Biophysical Chemistry·Homero Gómez-VelascoEnrique García-Hernández
Jul 10, 2019·ACS Applied Materials & Interfaces·Filipa GonçalvesArtur Cavaco-Paulo
Sep 5, 2021·Biological Reviews of the Cambridge Philosophical Society·Paolo Pelosi, Wolfgang Knoll

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