Crystal structures of CDC21-1 inteins from hyperthermophilic archaea reveal the selection mechanism for the highly conserved homing endonuclease insertion site

Extremophiles : Life Under Extreme Conditions
Hannes M BeyerHideo Iwaï

Abstract

Self-splicing inteins are mobile genetic elements invading host genes via nested homing endonuclease (HEN) domains. All HEN domains residing within inteins are inserted at a highly conserved insertion site. A purifying selection mechanism directing the location of the HEN insertion site has not yet been identified. In this work, we solved the three-dimensional crystal structures of two inteins inserted in the cell division control protein 21 of the hyperthermophilic archaea Pyrococcus abyssi and Pyrococcus horikoshii. A comparison between the structures provides the structural basis for the thermo-stabilization mechanism of inteins that have lost the HEN domain during evolution. The presence of an entire extein domain in the intein structure from Pyrococcus horikoshii suggests the selection mechanism for the highly conserved HEN insertion point.

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Citations

Nov 12, 2020·International Journal of Molecular Sciences·Hannes M BeyerHideo Iwaï
Mar 18, 2021·Biochimie·Sunita PandaSasmita Nayak
Jun 5, 2021·Scientific Reports·Jennie E WilliamsKenneth V Mills

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Methods Mentioned

BETA
X-ray
PCR

Software Mentioned

Refmac5
REDO
XDS
DIAMOND
MolProbity
Coot
ARP
MolProbity webserver
Phenix
WARP

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