Crystal structures of Escherichia coli RecA in a compressed helical filament

Journal of Molecular Biology
Xu Xing, C E Bell

Abstract

The X-ray crystal structure of uncomplexed Escherichia coli RecA protein has been determined in three new crystal forms at resolutions of 1.9 A, 2.0 A, and 2.6 A. The RecA protein used for this study contains the extra residues Gly-Ser-His-Met at the N terminus, but retains normal ssDNA-dependent ATPase and coprotease activities. In all three crystals, RecA is packed in a right-handed helical filament with a pitch of approximately 74 A. These RecA filaments are compressed relative to the original crystal structure of RecA, which has a helical pitch of 82.7 A. In the structures of the compressed RecA filament, the monomer-monomer interface and the core domain are essentially the same as in the RecA structure with the 83 A pitch. The change in helical pitch is accommodated by a small movement of the N-terminal domain, which is reoriented to preserve the contacts it makes at the monomer-monomer interface. The new crystal structures show significant variation in the orientation and conformation of the C-terminal domain, as well as in the inter-filament packing interactions. In crystal form 2, a calcium ion is bound closely to a beta-hairpin of the C-terminal domain and to Asp38 of a neighboring filament, and residues 329-331 of the...Continue Reading

References

Jan 1, 1979·Cold Spring Harbor Symposia on Quantitative Biology·J W RobertsE M Phizicky
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J W RobertsN L Craig
Jan 23, 1992·Nature·R M StoryT A Steitz
Jan 23, 1992·Nature·R M Story, T A Steitz
Jun 1, 1991·Journal of Structural Biology·X Yu, E H Egelman
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Jul 20, 1990·Journal of Molecular Biology·E DiCapuaP A Timmins
Nov 1, 1989·Analytical Biochemistry·S C Gill, P H von Hippel
Jul 28, 1989·Science·E H Egelman, X Yu
Oct 20, 1986·Journal of Molecular Biology·E H Egelman, A Stasiak
Dec 20, 1988·Journal of Molecular Biology·S L BrennerJ D Griffith
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J W Little
May 5, 1982·Journal of Molecular Biology·E Di CapuaT Koller
Sep 9, 1982·Nature·A Stasiak, E Di Capua
Jan 1, 1997·Progress in Nucleic Acid Research and Molecular Biology·A I Roca, M M Cox
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Oct 10, 1998·Molecular Microbiology·E N Zaitsev, S C Kowalczykowski
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·X YuE H Egelman
Sep 19, 2001·Biochemistry·K M LoganK L Knight
Aug 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Katsumi MorimatsuBengt Nordén
Feb 11, 2003·Structure·Margaret S VanLoockEdward H Egelman
Feb 22, 2003·The Journal of Biological Chemistry·Shelley L LusettiMichael M Cox
Feb 25, 2003·The Journal of Biological Chemistry·Shelley L LusettiMichael M Cox
Jul 3, 2003·Journal of Bacteriology·S DattaM Vijayan
Dec 25, 2003·Critical Reviews in Biochemistry and Molecular Biology·Dharia A McGrew, Kendall L Knight
Apr 14, 2004·Current Protein & Peptide Science·X YuE H Egelman
Jul 6, 2004·Nature Structural & Molecular Biology·Adam B ConwayPhoebe A Rice
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Jan 1, 1997·Methods in Enzymology·E A Merritt, D J Bacon
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

❮ Previous
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Citations

Jan 18, 2007·Nature Reviews. Molecular Cell Biology·Michael M Cox
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Digby F WarnerValerie Mizrahi
Mar 10, 2005·Nucleic Acids Research·Antonio ArizaCharles S Bond
May 11, 2006·Nucleic Acids Research·Rakhi RajanCharles E Bell
Dec 11, 2008·Nucleic Acids Research·Arabela A GrigorescuPhoebe A Rice
May 26, 2009·Acta Crystallographica. Section D, Biological Crystallography·Yang LiYu Luo
Jan 12, 2010·PloS One·Andrei L OkorokovElena V Orlova
Nov 18, 2008·Journal of Molecular Biology·Vitold E GalkinEdward H Egelman
Aug 29, 2006·Journal of Molecular Biology·Dieudonné Ndjonka, Charles E Bell
Jun 13, 2006·Structure·Vitold E GalkinEdward H Egelman
May 16, 2006·Structure·Melanie J BennettDavid Eisenberg
Jul 13, 2005·Cell·Dawit Kidane, Peter L Graumann
May 2, 2014·Nucleic Acids Research·Johnny LisboaSophie Quevillon-Cheruel
Jan 5, 2017·Journal of Biomolecular Structure & Dynamics·Anu V ChandranM Vijayan
Oct 1, 2005·Molecular Microbiology·Charles E Bell
Mar 17, 2007·Critical Reviews in Biochemistry and Molecular Biology·Michael M Cox
Sep 1, 2013·Biophysics Reviews·Anu V Chandran, M Vijayan
Dec 11, 2019·International Journal of Molecular Sciences·Dalton R Gibbs, Soma Dhakal
Feb 9, 2006·Chemical Reviews·Yoshizumi IshinoKosuke Morikawa

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