Crystal structures of fibronectin-binding sites from Staphylococcus aureus FnBPA in complex with fibronectin domains.

Proceedings of the National Academy of Sciences of the United States of America
Richard J BinghamJ R Potts

Abstract

Staphylococcus aureus can adhere to and invade endothelial cells by binding to the human protein fibronectin (Fn). FnBPA and FnBPB, cell wall-attached proteins from S. aureus, have multiple, intrinsically disordered, high-affinity binding repeats (FnBRs) for Fn. Here, 30 years after the first report of S. aureus/Fn interactions, we present four crystal structures that together comprise the structures of two complete FnBRs, each in complex with four of the N-terminal modules of Fn. Each approximately 40-residue FnBR forms antiparallel strands along the triple-stranded beta-sheets of four sequential F1 modules ((2-5)F1) with each FnBR/(2-5)F1 interface burying a total surface area of approximately 4,300 A(2). The structures reveal the roles of residues conserved between S. aureus and Streptococcus pyogenes FnBRs and show that there are few linker residues between FnBRs. The ability to form large intermolecular interfaces with relatively few residues has been proposed to be a feature of disordered proteins, and S. aureus/Fn interactions provide an unusual illustration of this efficiency.

References

Jul 5, 1992·Journal of Molecular Biology·J B MitchellS L Price
Jan 19, 1996·The Journal of Biological Chemistry·K House-PompeoM Höök
Aug 26, 1998·The New England Journal of Medicine·F D Lowy
Feb 2, 1999·Journal of Molecular Biology·L Lo ConteJ Janin
May 31, 2001·Journal of Molecular Graphics & Modelling·A K DunkerZ Obradovic
Feb 11, 2003·Trends in Biochemical Sciences·Kannan GunasekaranRuth Nussinov
May 9, 2003·Nature·Ulrich Schwarz-LinekJennifer R Potts
Jul 2, 2003·Acta Crystallographica. Section D, Biological Crystallography·Elizabeth PottertonEleanor Dodson
Jul 13, 2004·The Journal of Biological Chemistry·Ulrich Schwarz-LinekJennifer R Potts
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Sep 3, 2004·The EMBO Journal·Janet E DeaneJacqueline M Matthews
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Aug 12, 2005·Journal of Molecular Recognition : JMR·Vladimir N UverskyA Keith Dunker
Dec 22, 2005·Acta Crystallographica. Section D, Biological Crystallography·Andrew G W Leslie
Mar 18, 2006·The Journal of Infectious Diseases·Carlos ArrecubietaFranklin D Lowy
May 20, 2006·Nature Reviews. Microbiology·J Ross FitzgeraldDermot Cox
Jun 20, 2006·Microbes and Infection·Ulrich Schwarz-LinekJennifer R Potts
Jul 11, 2006·Trends in Biochemical Sciences·Han Remaut, Gabriel Waksman
Dec 6, 2006·Nucleic Acids Research·Megan SickmeierA Keith Dunker
Apr 28, 2007·The EMBO Journal·Ioannis VakonakisIain D Campbell
Jun 6, 2007·Thrombosis and Haemostasis·Giuseppe Lippi, Massimo Franchini
Jul 4, 2007·The Journal of Biological Chemistry·Nicola A G MeenanJennifer R Potts
Aug 3, 2007·International Journal of Antimicrobial Agents·A L CaseyT S J Elliott
Aug 8, 2007·Journal of Molecular Biology·Bálint MészárosZsuzsanna Dosztányi
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read

❮ Previous
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Citations

Dec 11, 2013·International Journal of Biological Macromolecules·D S Jemima BeulinKarthe Ponnuraj
Mar 9, 2012·Biochemistry·Galina TsurupaLeonid Medved
Mar 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Michèle C EratIoannis Vakonakis
Oct 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Steven K LowerVance G Fowler
Apr 16, 2009·The Journal of Biological Chemistry·Ioannis VakonakisIain D Campbell
Aug 27, 2010·The Journal of Biological Chemistry·Michèle C EratIoannis Vakonakis
Oct 16, 2010·The Journal of Biological Chemistry·Lisa M MaurerDeane F Mosher
Nov 10, 2010·The Journal of Biological Chemistry·Zoe R MarjenbergUlrich Schwarz-Linek
Nov 11, 2010·The Journal of Biological Chemistry·Yi-Pin LinYung-Fu Chang
Sep 29, 2011·The Journal of Biological Chemistry·Tomoo Ohashi, Harold P Erickson
Aug 16, 2011·The Journal of Biological Chemistry·Nicole C NorrisJennifer R Potts
Feb 22, 2012·The Journal of Biological Chemistry·Lisa M MaurerDeane F Mosher
May 9, 2013·The Journal of Biological Chemistry·Michèle C EratIoannis Vakonakis
Feb 7, 2013·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·D X Beringer, L M J Kroon-Batenburg
Apr 10, 2010·Microbiology and Immunology·Tsutomu YamasakiYusaku Nogami
Nov 3, 2009·Journal of Bacteriology·Michael E KonkelRebecca C Flanagan
Jan 30, 2010·BMC Evolutionary Biology·Sebastian Hoersch, Miguel A Andrade-Navarro
Jan 12, 2013·Current Protein & Peptide Science·James A Garnett, Steve Matthews
Mar 15, 2014·The Journal of Biological Chemistry·Vaclav StemberkJennifer R Potts
Jan 28, 2015·International Journal of Molecular Sciences·Daniel H Stones, Anne-Marie Krachler
May 13, 2011·European Journal of Cell Biology·Christine HoffmannChristof R Hauck
Apr 21, 2010·Experimental Cell Research·Ian R EllisSeth L Schor
Jul 30, 2015·Biomacromolecules·Roberto C Andresen EguiluzDelphine Gourdon
Mar 16, 2011·Protein Science : a Publication of the Protein Society·Krishnan Vengadesan, Sthanam V L Narayana
Nov 4, 2010·Biophysical Journal·Steven K LowerVance G Fowler

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