Crystal structures of MS2 capsids with mutations in the subunit FG loop

Journal of Molecular Biology
Nicola J StonehouseL Liljas

Abstract

The loop between the F and G beta strands (FG loop) of the bacteriophage MS2 coat protein subunit forms inter-subunit contacts around the 5-fold and 3-fold (quasi 6-fold) axes of the T=3 protein shell. In capsids, the loop is found in two very different conformations, one in B subunits, which form the 5-fold contact, and one in A and C subunits, which form the quasi 6-fold contact. One proline residue, Pro78, is strictly conserved in the coat protein of all related bacteriophages, and in the case of MS2 this proline residue is preceded by a cis peptide bond in the B subunit. In order to probe the role of the FG loop in capsid assembly, we have determined the crystal structures of two MS2 capsids, formed by coat proteins with mutations at two positions in the FG loop, P78N or E76D. These mutants show conformational changes in the FG loops that explain the reduced temperature stability of the capsids. The P78N mutant has a normal trans peptide bond at position 78.

Citations

Dec 1, 1996·Protein Science : a Publication of the Protein Society·C Z NiK R Ely
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Juan RegueraMauricio G Mateu
Jul 28, 2016·The Journal of Chemical Physics·Matthew R PerkettMichael F Hagan
Oct 7, 2016·Environmental Science & Technology·Qingxia ZhongTamar Kohn
May 15, 1996·Structure·R GolmohammadiL Liljas
Dec 23, 2003·European Journal of Biochemistry·Sheila M B LimaAndréa C de Oliveira
Jun 13, 2009·Protein Science : a Publication of the Protein Society·Pavel PlevkaLars Liljas
Aug 23, 2019·Food and Environmental Virology·Lin YaoYuxiu Zhai
Apr 1, 1996·Current Opinion in Structural Biology·G P Lomonossoff, J E Johnson
Mar 15, 1997·Structure·M BergdollP Dumas
Aug 15, 2019·Journal of Chemical Information and Modeling·Bo WangYinghao Wu
Apr 22, 2015·ACS Synthetic Biology·Jeff E GlasgowDanielle Tullman-Ercek
Sep 4, 2020·Biomacromolecules·Stephanie A RobinsonDanielle Tullman-Ercek

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