Crystal structures of the free and liganded form of an esterolytic catalytic antibody

Journal of Molecular Biology
G J WedemayerR C Stevens

Abstract

The crystal structure of the esterase catalytic antibody 48G7 has been determined in the presence of hapten at 2.0 A resolution and in the absence of hapten at 2.7 A resolution. The root-mean-square difference between the two structures is 0.6 A for the variable domain and 0.7 A for the constant domain. Comparison of the active site shows that no significant changes occur upon hapten binding as main-chain and side-chain displacements are negligible. Complex formation occurs as hapten fits into a pre-formed pocket about 10 A deep. Although 151 water molecules were modeled into the 48G7-hapten structure, none are bound in the active site. Comparison of the 48G7 structures with those of other published ester hydrolysis antibodies illustrates an emerging theme used by esterolytic antibodies in binding their (nitro-)phenyl haptens and in hydrolysing their cognate esters and carbonates: hapten is bound with the aryl end buried deep in the binding pocket, and the phosphonate moiety is responsible for the majority of the binding energy to the antibody-hapten interaction.

References

Sep 5, 1992·Journal of Molecular Biology·W R TulipP M Colman
Dec 30, 1991·Carbohydrate Research·G A Jeffrey, D B Huang
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Jul 1, 1990·Acta Crystallographica. Section A, Foundations of Crystallography·A T BrüngerJ W Erickson
Oct 5, 1990·Journal of Molecular Biology·J A IppolitoD W Christianson
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·E A PadlanD R Davies
Sep 20, 1987·Journal of Molecular Biology·S SheriffJ L Smith
Dec 19, 1986·Science·A TramontanoR A Lerner
Dec 19, 1986·Science·S J PollackP G Schultz
Dec 1, 1994·Current Opinion in Structural Biology·I A Wilson, R L Stanfield
Aug 20, 1993·Journal of Molecular Biology·M Shoham
Feb 11, 1994·Journal of Molecular Biology·L W GuddatA B Edmundson
Oct 15, 1993·Structure·R L StanfieldI A Wilson
Mar 15, 1994·Structure·B Golinelli-PimpaneauM Knossow
Sep 2, 1994·Journal of Molecular Biology·J H ArevaloI A Wilson
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S A LesleyP G Schultz
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·P D JeffreyS Sheriff
Jan 21, 1994·Journal of Molecular Biology·V A RobertsE D Getzoff
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J M RiniI A Wilson
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·V ChitarraR J Poljak
Feb 1, 1993·Protein Science : a Publication of the Protein Society·J F SchillbachM N Margolies
May 5, 1993·Journal of Molecular Biology·J H ArevaloI A Wilson
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·J B CharbonnierM Knossow
Feb 23, 1996·Science·P A PattenP G Schultz
May 28, 1996·Proceedings of the National Academy of Sciences of the United States of America·L C Hsieh-WilsonR C Stevens
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4

❮ Previous
Next ❯

Citations

Apr 18, 2008·The Journal of Biological Chemistry·Erik W DeblerIan A Wilson
Jun 5, 2012·Protein Engineering, Design & Selection : PEDS·Daisuke KurodaHaruki Nakamura
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·L T ChongP A Kollman
Aug 31, 2000·Annual Review of Biochemistry·D Hilvert
Jan 13, 2006·Structure·Justin GullingsrudJ Andrew McCammon
May 10, 2005·Journal of Molecular Recognition : JMR·Jean-Luc PellequerVictoria A Roberts
Dec 31, 2005·Physical Review Letters·Melik C Demirel, Arthur M Lesk
Aug 10, 1999·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·S OdaniF Gejyo
Jan 24, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tarique Khan, Dinakar M Salunke
Jun 26, 2013·Journal of the American Chemical Society·Sophie B SunFeng Wang
Oct 17, 1998·Biochemistry·F E RomesbergR C Stevens
Jul 11, 2000·Journal of Agricultural and Food Chemistry·Y W ChiuA E Karu
Dec 12, 2002·Chemical Reviews·Fujie Tanaka
Jun 13, 1997·Science·G J WedemayerR C Stevens

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.