Crystal structures of the lytic transglycosylase MltA from N.gonorrhoeae and E.coli: insights into interdomain movements and substrate binding.

Journal of Molecular Biology
Ailsa J PowellChristopher Davies

Abstract

MltA is a lytic transglycosylase of Gram-negative bacteria that cleaves the beta-1,4 glycosidic linkages between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) in peptidoglycan. We have determined the crystal structures of MltA from Neisseria gonorrhoeae and Escherichia coli (NgMltA and EcMltA), which have only 21.5% sequence identity. Both proteins have two main domains separated by a deep groove. Domain 1 shows structural similarity with the so-called double-psi barrel family of proteins. Comparison of the two structures reveals substantial differences in the relative positions of domains 1 and 2 such that the active site groove in NgMltA is much wider and appears more able to accommodate peptidoglycan substrate than EcMltA, suggesting that domain closure occurs after substrate binding. Docking of a peptidoglycan molecule into the structure of NgMltA reveals a number of conserved residues that are likely involved in substrate binding, including a potential binding pocket for the peptidyl moieties. This structure supports the assignment of Asp405 as the acid catalyst responsible for cleavage of the glycosidic bond. In EcMltA, the equivalent residue is Asp328, which has been identified previously. The structures...Continue Reading

References

Apr 18, 1967·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·C C BlakeV R Sarma
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·H SchindelinU Heinemann
Nov 1, 1995·Trends in Biochemical Sciences·L Holm, C Sander
Jan 15, 1997·The Biochemical Journal·G J DaviesB Henrissat
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·T C Terwilliger, J Berendzen
Oct 26, 1999·Acta Crystallographica. Section D, Biological Crystallography·J W Pflugrath
Jan 4, 2001·Journal of Molecular Evolution·N T Blackburn, A J Clarke
Jun 22, 2001·Acta Crystallographica. Section D, Biological Crystallography·R FedorovS Nikonov
Aug 3, 2002·European Journal of Biochemistry·Gary T JenningsGuido Grandi
Oct 24, 2002·Acta Crystallographica. Section D, Biological Crystallography·Thomas C Terwilliger
Nov 20, 2003·Cellular and Molecular Life Sciences : CMLS·G Koraimann
Mar 25, 2004·Acta Crystallographica. Section D, Biological Crystallography·Karin E Van StraatenAndy Mark W H Thunnissen
Nov 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·E A Merritt, M E Murphy
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Mar 9, 2005·Molecular Microbiology·Holly L HamiltonJoseph P Dillard
Apr 29, 2005·Acta Crystallographica. Section D, Biological Crystallography·Zhi-Jie LiuBi-Cheng Wang
Sep 6, 2005·Journal of Molecular Biology·Karin E van StraatenAndy-Mark W H Thunnissen

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Citations

Aug 15, 2008·Journal of the American Chemical Society·Maxim SuvorovShahriar Mobashery
Mar 22, 2012·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Yolande A ChanJoseph P Dillard
Jan 6, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Cecilia Artola-RecolonsJuan A Hermoso
Jan 9, 2013·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Lucille MoynieGunter Schneider
Dec 1, 2011·Microbiology and Molecular Biology Reviews : MMBR·Jean van Heijenoort
Jul 15, 2015·BMC Structural Biology·Doris H X QuayNicholas H Keep
Feb 13, 2008·FEMS Microbiology Reviews·Waldemar VollmerSimon Foster
Jul 10, 2019·Molecular Microbiology·Anna I WeaverTobias Dörr
Dec 9, 2008·International Journal of Palliative Nursing·Julia Downing
Nov 13, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Sanjita BanerjeeJudith S Bond
May 16, 2007·The Journal of Biological Chemistry·Karin E van StraatenAndy-Mark W H Thunnissen
Nov 21, 2012·Annals of the New York Academy of Sciences·Jarrod W JohnsonShahriar Mobashery
Aug 1, 2019·Scientific Reports·Christopher H JenkinsGalina V Mukamolova
Jun 24, 2017·Critical Reviews in Biochemistry and Molecular Biology·David A DikShahriar Mobashery

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