Crystal structures of two cyanobacterial response regulators in apo- and phosphorylated form reveal a novel dimerization motif of phytochrome-associated response regulators

Biophysical Journal
C BendaWolfgang Gärtner

Abstract

The structures of two response regulators (RRs) from the cyanobacterium Calothrix PCC7601, RcpA and RcpB, were solved to 1.9- and 1.75-A resolution, respectively. RcpA was found in phosphorylated and RcpB in nonphosphorylated form. Both RRs are members of phytochrome-associated, light-sensing two-component signal transduction pathways, based on histidine kinase-mediated receptor autophosphorylation and phosphorelay to a RR. Despite the overall folding similarity to CheY-type RRs ((beta/alpha)(5)-motif), RcpA and RcpB form homodimers, irrespective of their phosphorylation state, giving insight into a signal transduction putatively different from that of other known RRs. Dimerization is accomplished by a C-terminal extension of the RR polypeptide chain, and the surface formed by H4, beta 5, and H5, which constitute a hydrophobic contact area with distinct interactions between residues of either subunit. Sequence alignments reveal that the identified dimerization motif is archetypal for phytochrome-associated RRs, making them a novel subgroup of CheY-type RRs. The protein structures of RcpA and RcpB are compared to the recently presented protein structure of Rcp1 from Synechocystis.

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Citations

Apr 9, 2005·Trends in Microbiology·Klaas J Hellingwerf
Oct 8, 2009·The Journal of Biological Chemistry·Shivani ShardaWolfgang Gärtner
Dec 3, 2005·Journal of Bacteriology·Kottayil I Varughese
Jan 19, 2010·Current Opinion in Microbiology·Rong Gao, Ann M Stock
Oct 28, 2006·Current Opinion in Microbiology·Janina PurschwitzReinhard Fischer
May 18, 2012·Protein Science : a Publication of the Protein Society·Petya Violinova KrastevaHolger Sondermann
Jul 11, 2012·The Plant Journal : for Cell and Molecular Biology·Manikandan VeerabaguVirtudes Mira-Rodado
Aug 16, 2016·Journal of Molecular Biology·Christopher P ZschiedrichHendrik Szurmant
Feb 3, 2016·Journal of Bacteriology·Anna W BakerKatrina T Forest
Mar 7, 2007·Science Progress·Martijn BekkerKlaas J Hellingwerf
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Oct 13, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Xuefei YangXiaojing Yang

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