Crystallization and avoiding the problem of hemihedral twinning in crystals of Delta1-pyrroline-5-carboxylate dehydrogenase from Thermus thermophilus

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Eiji InagakiTahir H Tahirov

Abstract

Delta1-Pyrroline-5-carboxylate dehydrogenase from Thermus thermophilus (TtP5CDh) has been crystallized in a citrate-bound form (TtP5CDh-cit). The crystals diffracted to well beyond 2 A resolution, but exhibited perfect or near-perfect hemihedral twinning. Variation of crystallization conditions resulted in the growth of larger untwinned crystals or crystals with significantly reduced twin content, all with similar unit-cell parameters. The soaking of TtP5CDh-cit crystals in citrate-free solution produced crystals of the apo form (TtP5CDh-apo). The TtP5CDh-apo crystals belong to space group R3, with unit-cell parameters a = b = 102.29, c = 279.28 A, and diffract to 1.08 A. Crystals soaked in solution with NAD+ (TtP5CDh-NAD), NADH (TtP5CDh-NADH) and glutamate (TtP5CDh-Glu) were also prepared and characterized.

References

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Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Oct 29, 1998·Protein Science : a Publication of the Protein Society·K JohanssonH Eklund
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Feb 27, 2003·Chemico-biological Interactions·Nickolas A Sophos, Vasilis Vasiliou
Dec 20, 2003·Acta Crystallographica. Section D, Biological Crystallography·Hitomi TakahashiTahir H Tahirov
Sep 25, 2004·Acta Crystallographica. Section D, Biological Crystallography·Hitomi TakahashiTahir H Tahirov

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Citations

Jun 8, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Eiji InagakiTahir H Tahirov
Aug 29, 2006·Journal of Molecular Biology·Eiji InagakiTahir H Tahirov

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