Crystallization and initial X-ray diffraction studies of scaffolding protein (gp7) of bacteriophage phi29

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Mohammed O Badasso, Dwight L Anderson

Abstract

The Bacillus subtilis bacteriophage phi29 scaffolding protein (gp7) has been crystallized by the hanging-drop vapour-diffusion method at 293 K. Two new distinct crystal forms that both differed from a previously crystallized and solved scaffolding protein were grown under the same conditions. Form I belongs to the primitive tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 77.13, c = 37.12 A. Form II crystals exhibit an orthorhombic crystal form, with space group C222 and unit-cell parameters a = 107.50, b = 107. 80, c = 37.34 A. Complete data sets have been collected to 1.78 and 1.80 A for forms I and II, respectively, at 100 K using Cu Kalpha X-rays from a rotating-anode generator. Calculation of a VM value of 2.46 A3 Da(-1) for form I suggests the presence of one molecule in the asymmetric unit, corresponding to a solvent content of 50.90%, whereas form II has a VM of 4.80 A3 Da(-1) with a solvent content of 48.76% and two molecules in the asymmetric unit. The structures of both crystal forms are being determined by the molecular-replacement method using the coordinates of the published crystal structure of gp7.

References

Jun 20, 1990·European Journal of Biochemistry·E Kellenberger
Jan 1, 1985·Annual Review of Microbiology·C Bazinet, J King
Jul 5, 1988·Journal of Molecular Biology·C Bazinet, J King
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Nov 15, 1981·Journal of Molecular Biology·R A Driedonks van Driel
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
May 18, 1999·Journal of Molecular Biology·M ValleJ L Carrascosa
Oct 26, 1999·Acta Crystallographica. Section D, Biological Crystallography·J W Pflugrath
Aug 25, 2000·Acta Crystallographica. Section D, Biological Crystallography·M O BadassoD Anderson
Jun 5, 2003·Nature Structural Biology·Marc C MoraisMichael G Rossmann

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Related Papers

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Yuko OnoharaTadashi Yoshimoto
Acta Crystallographica. Section D, Biological Crystallography
Masatake AkitaKoki Horikoshi
Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Haruka TamuraYasushi Kai
Acta Crystallographica. Section D, Biological Crystallography
Mark A WhiteRobert O Fox
Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Haruka TamuraHiroyoshi Matsumura
© 2021 Meta ULC. All rights reserved