Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis

Acta Crystallographica. Section D, Biological Crystallography
Christopher G MowatMalcolm D Walkinshaw

Abstract

The product of the Rv2276 gene of Mycobacterium tuberculosis is a cytochrome P450 (P450 MT2, CYP121) which has been shown to bind tightly to a range of azole-based antifungal drugs (e.g. miconazole, clotrimazole). These drugs are potent inhibitors of mycobacterial growth, suggesting that P450 MT2 (CYP121) may be a potential drug target. The enzyme has been overexpressed in Escherichia coli and crystallized by the hanging-drop method. Crystals of P450 MT2 (CYP121) belong to the hexagonal space group P6(1)22 or P6(5)22, with unit-cell parameters a = b = 78.3, c = 265.6 A. Native data have been collected to 1.6 A resolution and Hg-derivative data to 2.5 A resolution using a synchrotron-radiation source.

Citations

May 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Pascal BelinMuriel Gondry
Dec 2, 2004·Proteins·David LaDennis R Livesay
Nov 19, 2002·The Journal of Biological Chemistry·David LeysAndrew W Munro
Oct 1, 2008·Journal of Enzyme Inhibition and Medicinal Chemistry·Ahmet OzdemirYağmur Tunali

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