Crystallization and preliminary crystallographic analysis of an aminoglycoside kinase from Legionella pneumophila

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Christopher T LemkeAlbert M Berghuis

Abstract

9-Aminoglycoside phosphotransferase type Ia [APH(9)-Ia] is a resistance factor in Legionella pneuemophila, the causative agent of legionnaires' disease. It is responsible for providing intrinsic resistance to the antibiotic spectinomycin. APH(9)-Ia phosphorylates one of the hydroxyl moieties of spectinomycin in an ATP-dependent manner, abolishing the antibiotic properties of this drug. Here, the crystallization and preliminary X-ray studies of this enzyme in two crystal forms is reported. One of the these crystal forms provides diffraction data to a resolution of 1.7 A.

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Citations

Jan 22, 2010·The Journal of Biological Chemistry·Desiree H FongAlbert M Berghuis
Aug 3, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Daisuke IinoShunsuke Yajima

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