Abstract
Beta-D-Xylosidases (EC 3.2.1.37) are hemicellulases that cleave single xylose units from the nonreducing end of xylooligomers. In this study, the crystallization and preliminary X-ray analysis of a beta-D-xylosidase from Geobacillus stearothermophilus T-6 (XynB3), a family 43 glycoside hydrolase, is described. XynB3 is a 535-amino-acid protein with a calculated molecular weight of 61 891 Da. Purified recombinant native and catalytic inactive mutant proteins were crystallized and cocrystallized with xylobiose in two different space groups, P2(1)2(1)2 (unit-cell parameters a = 98.32, b = 99.36, c = 258.64 A) and P4(1)2(1)2 (or the enantiomorphic space group P4(3)2(1)2; unit-cell parameters a = b = 140.15, c = 233.11 A), depending on the detergent. Transferring crystals to cryoconditions required a very careful protocol. Orthorhombic crystals diffract to 2.5 A and tetragonal crystals to 2.2 A.
References
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·B HenrissatG Davies
Nov 15, 1996·Journal of Biotechnology·A LapidotY Shoham
Sep 11, 2001·Applied Microbiology and Biotechnology·Q K BegG S Hoondal
Apr 12, 2002·FEBS Letters·Dalia ShallomYuval Shoham
Aug 29, 2002·Nature Structural Biology·Didier NurizzoGideon J Davies
Sep 11, 2002·The Journal of Biological Chemistry·Dalia ShallomYuval Shoham
May 10, 2003·The Journal of Biological Chemistry·Tsafrir BravmanYuval Shoham
Jul 2, 2003·Current Opinion in Microbiology·Dalia Shallom, Yuval Shoham
Sep 3, 2003·Biochemistry·Tsafrir BravmanYuval Shoham
Oct 1, 2003·The EMBO Journal·Klaus HövelDietmar Schomburg
Oct 24, 2003·The Journal of Biological Chemistry·Gali GolanGil Shoham
Dec 17, 2003·Methods in Enzymology·Elspeth F Garman, Sylvie Doublié
Jan 30, 2004·Proteins·Tirso PonsLázaro Hernández
Feb 20, 2004·The Journal of Biological Chemistry·François AlbertoMirjam Czjzek
Apr 23, 2004·Acta Crystallographica. Section D, Biological Crystallography·A TeplitskyG Shoham
Jul 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Gennady ZolotnitskyYuval Shoham
Oct 7, 2004·Journal of Bacteriology·Dalia ShallomYuval Shoham
Jan 5, 2005·Biochemistry·Dalia ShallomYuval Shoham
Jan 18, 2005·FEMS Microbiology Reviews·Tony CollinsGeorges Feller
Oct 11, 2005·Journal of Molecular Biology·Mirjam CzjzekYuval Shoham
Citations
Oct 15, 2013·Journal of Industrial Microbiology & Biotechnology·Zongqing HuangZiduo Liu
Apr 25, 2006·Journal of Molecular Biology·Christian BrüxDietmar Schomburg
Nov 6, 2014·Acta Crystallographica. Section D, Biological Crystallography·Shifra LanskyGil Shoham
Jun 15, 2019·Biotechnology for Biofuels·Mohamed MrouehSandrine Pagès