Crystallization and preliminary crystallographic study of the yeast Malassezia sympodialis allergen Mala s 1

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Monica VilhelmssonAdnane Achour

Abstract

The opportunistic yeast Malassezia sympodialis can act as an allergen and elicit specific IgE- and T-cell reactivity in patients with atopic eczema. The first identified major allergen from M. sympodialis, Mala s 1, is present on the cell surface of the yeast. Recombinant Mala s 1 was expressed in Escherichia coli, purified and refolded in a soluble form. Crystals of Mala s 1 were obtained in 25% PEG 8K, 0.2 M (NH4)2SO4. Crystals belong to space group P2(1)2(1)2, with unit-cell parameters a = 44.4, b = 163.7, c = 50.6 A, and diffract to 1.35 A resolution.

References

May 18, 1999·The Journal of Allergy and Clinical Immunology·A ZargariP Whitley
Oct 26, 1999·Acta Crystallographica. Section D, Biological Crystallography·D Bourgeois
Apr 30, 2002·International Archives of Allergy and Immunology·Annika ScheyniusMaria Tengvall Linder
Jun 5, 2003·Acta Crystallographica. Section D, Biological Crystallography·Liselotte KaiserAdnane Achour
Jul 10, 2003·The Journal of Biological Chemistry·Liselotte KaiserGunter Schneider
Mar 3, 2004·The Journal of Clinical Investigation·Donald Y M LeungQutayba A Hamid

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Citations

May 19, 2007·Medical Mycology·H R Ashbee

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