Crystallization and preliminary crystallographic analysis of human glycosylated haemoglobin

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Vitaly E SyakhovichDino Moras

Abstract

Human glycosylated haemoglobin A1C is a stable minor variant formed in vivo by post-translational modification of the main form of haemoglobin by glucose. Crystals of oxyHbA1C were obtained using the hanging-drop vapour-diffusion method and PEG as precipitant. The diffraction pattern of the crystal extends to a resolution of 2.3 A at 120 K. The crystals belong to space group C2, with unit-cell parameters a = 237.98, b = 59.27, c = 137.02 A, alpha = 90.00, beta = 125.40, gamma = 90.00 degrees. The presence of two and a half molecules per asymmetric unit gives a crystal volume per protein weight (VM) of 9.70 A3 Da(-1) and a solvent content of 49%.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C E GuthrowJ W Baynes
Nov 3, 1975·Biochemical and Biophysical Research Communications·H F BunnP M Gallop
Jul 11, 1972·Biochemical and Biophysical Research Communications·S P Robins, A J Bailey
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S K WilliamsM W Bitensky
Apr 1, 1997·Cardiovascular Research·C L OltmanK C Dellsperger
Apr 15, 2004·Biochemical and Biophysical Research Communications·Vitaly E SyakhovichSergey B Bokut

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Citations

Sep 2, 2015·Journal of Biomolecular Structure & Dynamics·Govindarajan Prasanna, N T Saraswathi
Oct 11, 2019·The FEBS Journal·Monita MuralidharanAmit Kumar Mandal

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