Crystallization and preliminary crystallographic studies of dihydrofolate reductase-thymidylate synthase from Trypanosoma cruzi, the Chagas disease pathogen.

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Penchit ChitnumsubYongyuth Yuthavong

Abstract

Trypanosoma cruzi dihydrofolate reductase-thymidylate synthase (TcDHFR-TS) was crystallized in complexes with the dihydrotriazine-based or quinazoline-based antifolates C-448, cycloguanil (CYC) and Q-8 in order to gain insight into the interactions of this DHFR enzyme with classical and novel inhibitors. The TcDHFR-TS-C-448-NDP-dUMP crystal belonged to space group C222(1) with two molecules per asymmetric unit and diffracted to 2.37 angstrom resolution. The TcDHFR-TS-CYC, TcDHFR-TS-CYC-NDP and TcDHFR-TS-Q-8-NDP crystals belonged to space group P2(1) with four molecules per asymmetric unit and diffracted to 2.1, 2.6 and 2.8 angstrom resolution, respectively. Crystals belonging to the two different space groups were suitable for structure determination.

References

Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·A R BelloS M Beverley
Oct 27, 2001·Acta Crystallographica. Section D, Biological Crystallography·N SchormannD Chattopadhyay
Mar 5, 2003·Molecular and Biochemical Parasitology·Olga SenkovichDebasish Chattopadhyay
Nov 7, 2003·Lancet·Michael P BarrettSanjeev Krishna
Jan 23, 2004·Journal of Medicinal Chemistry·Sumalee KamchonwongpaisanYongyuth Yuthavong
Mar 25, 2004·Acta Crystallographica. Section D, Biological Crystallography·Penchit ChitnumsubYongyuth Yuthavong

❮ Previous
Next ❯

Citations

May 31, 2011·Expert Opinion on Therapeutic Patents·Dennis L Wright, Amy C Anderson
May 25, 2016·Biochimica Et Biophysica Acta. General Subjects·Kinga Nyíri, Beáta G Vértessy
Jun 10, 2011·ACS Chemical Biology·Jarunee VanichtanankulYongyuth Yuthavong

❮ Previous
Next ❯

Related Concepts

© 2022 Meta ULC. All rights reserved