Crystallization and preliminary structure analysis of the blue laccase from the ligninolytic fungus Panus tigrinus

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
M FerraroniF Briganti

Abstract

The blue laccase from the white-rot basidiomycete fungus Panus tigrinus, an enzyme involved in lignin biodegradation, has been crystallized. P. tigrinus laccase crystals grew within one week at 296 K using the sitting-drop vapour-diffusion method in 22%(w/v) PEG 4000, 0.2 M CaCl2, 100 mM Tris-HCl pH 7.5. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 54.2, b = 111.6, c = 97.1, beta = 97.7 degrees , and contain 46% solvent. A complete native data set was collected to 1.4 A resolution at the copper edge. Molecular replacement using the Coprinus cinereus laccase structure (PDB code 1hfu) as a starting model was performed and initial electron-density maps revealed the presence of a full complement of copper ions. Model refinement is in progress. The P. tigrinus laccase structural model exhibits the highest resolution available to date and will assist in further elucidation of the catalytic mechanism and electron-transfer processes for this class of enzymes.

References

Jan 26, 1990·European Journal of Biochemistry·A Messerschmidt, R Huber
Aug 1, 1993·Environmental Health Perspectives·S D Aust, J T Benson
Nov 22, 1997·FEMS Microbiology Letters·A A LeontievskyA Hatakka
Aug 12, 1999·Fungal Genetics and Biology : FG & B·A LeonowiczJ Rogalski
Feb 2, 2000·Applied and Environmental Microbiology·C Johannes, A Majcherczyk
Jul 16, 2002·Nature Structural Biology·Nina HakulinenJuha Rouvinen
Jul 20, 2002·Phytochemistry·Alfred M Mayer, Richard C Staples
Mar 15, 2003·The Journal of Biological Chemistry·Francisco J EnguitaMaria Arménia Carrondo
Feb 7, 2004·The Journal of Biological Chemistry·Francisco J EnguitaMaria Arménia Carrondo
Mar 24, 2004·Micron : the International Research and Review Journal for Microscopy·Harald Claus

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Citations

Apr 11, 2009·Biotechnology Letters·Kristiina HildénTaina Lundell
Jun 11, 2009·Applied Biochemistry and Biotechnology·Daljit Singh Arora, Rakesh Kumar Sharma
Aug 9, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Andrey V LyashenkoAl'bert M Mikhailov
Nov 21, 2007·Biochemistry. Biokhimii︠a︡·O V MorozovaA I Yaropolov
Apr 16, 2014·Enzyme and Microbial Technology·Maurycy DarochLesley A Iwanejko
Dec 4, 2012·Enzyme and Microbial Technology·Natalia PozdnyakovaAnna Jarosz-Wilkolazka
Nov 26, 2016·Microbes and Environments·Nobutada Kimura, Yoichi Kamagata

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