Crystallization and preliminary X-ray analysis of gene product 44 from bacteriophage Mu

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Youhei KondouTomitake Tsukihara

Abstract

Bacteriophage Mu baseplate protein gene product 44 (gp44) is an essential protein required for the assembly of viable phages. To investigate the roles of gp44 in baseplate assembly and infection, gp44 was crystallized at pH 6.0 in the presence of 20% 2-methyl-2,4-pentanediol. The crystals belong to space group R3, with unit-cell parameters a = b = 127.47, c = 63.97 A. The crystals diffract X-rays to at least 2.1 A resolution and are stable in the X-ray beam and are therefore appropriate for structure determination. Native data have been collected to 2.1 A resolution using a DIP6040 image-plate system at beamline BL44XU at the SPring-8 facility in Japan.

References

Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Aug 19, 2003·Nature Structural Biology·Victor A KostyuchenkoMichael G Rossmann
Nov 20, 2003·Cellular and Molecular Life Sciences : CMLS·P G LeimanM G Rossmann

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Citations

Sep 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Stefan PukatzkiJohn J Mekalanos
Aug 30, 2005·Journal of Molecular Biology·Youhei KondouTomitake Tsukihara
Jun 10, 2005·Journal of Biochemistry·Daisuke KitazawaHarumi Fukada

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