Crystallization and preliminary X-ray analysis of alginate lyases A1-II and A1-II' from Sphingomonas sp. A1

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Masayuki YamasakiB Mikami

Abstract

Alginate lyases depolymerize alginate, a heteropolysaccharide consisting of alpha-L-guluronate and beta-D-mannuronate, through a beta-elimination reaction. The alginate lyases A1-II (25 kDa) and A1-II' (25 kDa) from Sphingomonas sp. A1, which belong to polysaccharide lyase family PL-7, exhibit 68% homology in primary structure but have different substrate specificities. To determine clearly the structural basis for substrate recognition in the depolymerization mechanism by alginate lyases, both proteins were crystallized at 293 K using the vapour-diffusion method. A crystal of A1-II belonged to space group P2(1) and diffracted to 2.2 A resolution, with unit-cell parameters a = 51.3, b = 30.1, c = 101.6 A, beta = 100.2 degrees, while a crystal of A1-II' belonged to space group P2(1)2(1)2(1) and diffracted to 1.0 A resolution, with unit-cell parameters a = 34.6, b = 68.5, c = 80.3 A.

References

Feb 1, 1992·Bioscience, Biotechnology, and Biochemistry·H AkiyamaK Okayama
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Aug 6, 1998·Bioscience, Biotechnology, and Biochemistry·Y MatsubaraT Muramatsu
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Apr 6, 2002·Journal of Wound Care·S Thomas

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Citations

Apr 26, 2005·Bioscience, Biotechnology, and Biochemistry·Wataru HashimotoKousaku Murata
Jun 3, 2008·Journal of Molecular Biology·Kohei OguraKousaku Murata
Aug 6, 2005·Journal of Molecular Biology·Masayuki YamasakiKousaku Murata

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