Crystallization and preliminary X-ray analysis of reducing-end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Yuji HondaMotomitsu Kitaoka

Abstract

The reducing-end xylose-releasing exo-oligoxylanase (Rex) from Bacillus halodurans C-125, a novel family GH8 glycoside hydrolase, was crystallized by the hanging-drop vapour-diffusion method using 13.6 mg ml(-1) purified Rex, 5.6%(v/v) polyethylene glycol 4000, 70 mM sodium acetate pH 4.6 and 30%(v/v) glycerol. Suitable crystals grew after incubation for 5 d at 293 K. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 52.69, b = 86.02, c = 87.92 A. X-ray diffraction data were collected at a resolution of 1.35 A.

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Citations

Oct 13, 2009·Journal of Biochemistry·Masafumi HidakaMotomitsu Kitaoka
Feb 19, 2005·The Journal of Biological Chemistry·Shinya FushinobuMotomitsu Kitaoka
Nov 23, 2005·The Journal of Biological Chemistry·Yuji Honda, Motomitsu Kitaoka

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