Crystallization and preliminary X-ray analysis of (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC) synthase (MenD) from Escherichia coli

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Edyta A L SieminskaDavid A R Sanders

Abstract

(1R,6R)-2-Succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC) synthase, also called MenD, participates in the menaquinone (vitamin K2) biosynthetic pathway. The enzyme is a part of the superfamily of ThDP-dependent enzymes; however, it is the only enzyme known to catalyze a Stetter-like 1,4-addition of a ThDP adduct to the beta-carbon of an unsaturated carboxylate. This is the first reported crystallization of the apoenzyme and holoenzyme forms of MenD. The apoenzyme crystals were obtained by sitting-drop vapour diffusion with 70% MPD. However, the crystals were too small to collect diffraction data and a search for better conditions was not successful. Single crystals of the holoenzyme with ThDP and Mn2+ as cofactors were obtained by the hanging-drop vapour-diffusion method with 35% ethylene glycol as precipitant. Diffraction data were collected on a cryocooled crystal to a resolution of 2.0 A at BioCARS, Advanced Photon Source (APS), Chicago, IL, USA. The crystal was found to belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 106.86, b = 143.06, c = 156.85 A, alpha = beta = gamma = 90 degrees.

References

Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Sep 22, 2001·Acta Crystallographica. Section D, Biological Crystallography·R J Read
Mar 21, 2002·Journal of Molecular Biology·Siew Siew PangLuke W Guddat

❮ Previous
Next ❯

Citations

Apr 3, 2009·Biochemical and Biophysical Research Communications·Amit PriyadarshiKwang Yeon Hwang
Aug 1, 2009·EcoSal Plus·R Meganathan, Ohsuk Kwon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.