Crystallization and preliminary X-ray analysis of cryptochrome 3 from Arabidopsis thaliana

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
R PokornyA Batschauer

Abstract

Cryptochromes are flavoproteins which serve as blue-light receptors in plants, animals, fungi and prokaryotes and belong to the same protein family as the catalytically active DNA photolyases. Cryptochrome 3 from the plant Arabidopsis thaliana (cry3; 525 amino acids, 60.7 kDa) is a representative of the novel cryDASH subfamily of UV-A/blue-light receptors and has been expressed as a mature FAD-containing protein in Escherichia coli without the signal sequence that directs the protein into plant organelles. The purified cryptochrome was found to be complexed to methenyltetrahydrofolate as an antenna pigment. Crystals of the cryptochrome-antenna pigment complex were obtained by vapour diffusion and display orthorhombic symmetry, with unit-cell parameters a = 76.298, b = 116.782, c = 135.024 A. X-ray diffraction data were collected to 1.9 A resolution using synchrotron radiation. The asymmetric unit comprises a cry3 dimer, the physiological role of which remains to be elucidated.

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Citations

Nov 15, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yihua HuangJohann Deisenhofer
Jun 18, 2009·The Journal of Biological Chemistry·Julia MoldtAlfred Batschauer
Dec 22, 2015·MicrobiologyOpen·Annika BrychAlfred Batschauer
Oct 6, 2009·Journal of Photochemistry and Photobiology. B, Biology·P ZirakL-O Essen
Jul 29, 2015·Photochemistry and Photobiology·Marta CastrilloRichard Pokorny
May 27, 2006·Journal of Photochemistry and Photobiology. B, Biology·S-H SongL-O Essen
Jul 9, 2016·Journal of Photochemistry and Photobiology. B, Biology·Parul PariharSheo Mohan Prasad
Oct 20, 2006·Chembiochem : a European Journal of Chemical Biology·Tobias KlarLars-Oliver Essen
Sep 1, 2017·Photochemistry and Photobiology·Elisabeth IgnatzLars-Oliver Essen
Feb 23, 2020·Biochemistry. Biokhimii︠a︡·Yu L VechtomovaM S Kritsky
Sep 19, 2020·Current Biology : CB·Eusebio NavarroVictoriano Garre

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