Crystallization and preliminary X-ray analysis of the isomerase domain of glucosamine-6-phosphate synthase from Candida albicans

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Jarosław OlchowyWojciech Rypniewski

Abstract

Glucosamine-6-phosphate synthase (EC 2.6.1.16) catalyses the first and practically irreversible step in the hexosamine metabolism pathway, the end product of which, uridine 5'-diphospho-N-acetyl D-glucosamine, is an essential substrate for assembly of the cell wall. The isomerase domain, consisting of residues 346-712 (42 kDa), of glucosamine-6-phosphate synthase from Candida albicans has been crystallized. X-ray analysis revealed that the crystals belonged to space group I4, with unit-cell parameters a = b = 149, c = 103 A. Diffraction data were collected to 3.8 A. Preliminary results from molecular replacement using the homologous bacterial monomer reveal that the asymmetric unit contains two monomers that resemble a bacterial dimer. The crystal lattice consists of pairs of such symmetry-related dimers forming elongated tetramers.

References

Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Feb 6, 1999·The Journal of Biological Chemistry·S MilewskiG W Gooday
Mar 26, 1999·Protein Science : a Publication of the Protein Society·A TeplyakovB Badet
Nov 9, 2001·Journal of Molecular Biology·A TeplyakovM A Badet-Denisot
Jun 5, 2002·Biochimica Et Biophysica Acta·Sławomir Milewski
Apr 5, 2005·Acta Crystallographica. Section D, Biological Crystallography·Airlie J McCoyRandy J Read

❮ Previous
Next ❯

Citations

Aug 8, 2007·Journal of Molecular Biology·Joanna RaczynskaWojciech Rypniewski
Feb 21, 2007·The Biochemical Journal·Jarosław OlchowySławomir Milewski

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

© 2021 Meta ULC. All rights reserved