Abstract
Infection by the parasite Plasmodium falciparum is the leading cause of malaria in humans. The parasite has a unique and essential plastid-like organelle called the apicoplast. The apicoplast contains a genome that undergoes replication and repair through the action of a replicative polymerase (apPOL). apPOL has no direct orthologs in mammalian polymerases and is therefore an attractive antimalarial drug target. No structural information exists for apPOL, and the Klenow fragment of Escherichia coli DNA polymerase I, which is its closest structural homolog, shares only 28% sequence identity. Here, conditions for the crystallization of and preliminary X-ray diffraction data from crystals of P. falciparum apPOL are reported. Data complete to 3.5 Å resolution were collected from a single crystal (2 × 2 × 5 µm) using a 5 µm beam. The space group P6522 (unit-cell parameters a = b = 141.8, c = 149.7 Å, α = β = 90, γ = 120°) was confirmed by molecular replacement. Refinement is in progress.
References
Apr 16, 1993·Science·L S BeeseT A Steitz
Jan 15, 1999·Biochemistry·C A BrautigamT A Steitz
Jun 1, 2005·Molecular and Biochemical Parasitology·Fiona SeowMichael P Barrett
Mar 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Robin Leslie OwenElspeth F Garman
Jul 21, 2006·Acta Crystallographica. Section D, Biological Crystallography·Wladek MinorMaksymilian Chruszcz
Feb 26, 2009·Journal of Synchrotron Radiation·Robert F FischettiJanet L Smith
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
May 13, 2010·International Review of Cell and Molecular Biology·Frank Seeber, Dominique Soldati-Favre
May 25, 2010·Biochemical Society Transactions·Ming Kalanon, Geoffrey I McFadden
May 3, 2011·FEBS Letters·Ines PetersenMichael Lanzer
May 3, 2011·Nature·Frank DiMaioDavid Baker
Oct 24, 2013·Biochemistry·Bentley M WingertScott W Nelson
Apr 2, 2014·Journal of Biomolecular Screening·Morgan E MillerScott W Nelson