Crystallization and preliminary X-ray analysis of neuropsin, a serine protease expressed in the limbic system of mouse brain

Journal of Structural Biology
T KishiT Hakoshima

Abstract

Neuropsin (M(r) 25032) is a serine protease expressed in the limbic system of mouse brain. It has been implicated in various neurological processes including formation of memory and may be important as a drug target in the treatment of epilepsy. The recombinant protein was produced using a baculovirus expression system and was purified. Two crystal forms were obtained by a hanging-drop vapor-diffusion method with polyethylene glycol. Preliminary X-ray crystallographic analysis revealed that crystal form I belongs to triclinic space group P1 with unit cell dimensions a = 97.16 A, b = 97.12 A, c = 46.75 A and alpha = 99.17 degrees, beta = 99.77 degrees, gamma = 117.35 degrees. Self-rotation function analysis of these data of form I indicates the position of a noncrystallographic threefold axis. There are six molecules in the crystallographic asymmetric unit. Crystal form II also belongs to triclinic space group P1 but has unit cell dimensions of a = 38.40 A, b = 55.16 A, c = 65.37 A and alpha = 95.38 degrees, beta = 89.98 degrees, gamma = 110.46 degrees with two molecules in the crystallographic asymmetric unit. Form II has a noncrystallographic twofold axis. Intensity data to 3.1 A resolution for form I and to 2.2 A resolution f...Continue Reading

References

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Citations

Apr 10, 2007·Biochemistry·Sachiko I BlaberMichael Blaber
Jun 28, 2006·Biological Chemistry·Nathalie Heuzé-Vourc'hYves Courty
Feb 5, 2010·Biological Chemistry·Michael BlaberSachiko I Blaber
Jul 11, 2006·Journal of Neuroimmunology·I A ScarisbrickG P Christophi
Feb 6, 1999·The Journal of Biological Chemistry·T KishiT Hakoshima

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