Crystallization and preliminary X-ray diffraction studies of the epsilonzeta addiction system encoded by Streptococcus pyogenes plasmid pSM19035

Acta Crystallographica. Section D, Biological Crystallography
Anton MeinhartWolfram Saenger

Abstract

The proteins encoded by the Streptococcus pyogenes broad-host range and low copy-number plasmid pSM19035 form a toxin-antitoxin module that secures stable maintenance by causing the death of plasmid-free segregants. The epsilonzeta protein complex was crystallized in four different forms at pH 5.0 and pH 7.0 using the vapour-diffusion method with PEG 3350 and ethylene glycol as precipitants. Three of the crystal forms were obtained in the same droplet under identical conditions at pH 5.0. One form belongs to the enantiomorphic space groups P4(3)2(1)2 or P4(1)2(1)2. For the other two, the X-ray reflection conditions match those of space group P2(1)2(1)2(1), one representing a superlattice of the other. A crystal form growing at pH 7.0 also belongs to space group P2(1)2(1)2(1), but there is no indication of a structural relationship to the other orthorhombic forms. Initially, the crystals diffracted to 2.9 A resolution and diffracted to 1.95 A after soaking at pH 7.0. A preparation of selenomethionyl epsilonzeta protein complex yielded single crystals suitable for X-ray diffraction experiments using synchrotron sources.

Citations

Aug 9, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Hannes Mutschler, Anton Meinhart
Apr 20, 2004·Journal of Bacteriology·Jeremy Allen Smith, Roy David Magnuson
Mar 28, 2009·PLoS Genetics·Laurence Van Melderen, Manuel Saavedra De Bast
Feb 2, 2012·PloS One·Virginia S LioyJuan C Alonso
Feb 4, 2014·PloS One·Mariangela TaboneJuan C Alonso
Feb 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Anton MeinhartWolfram Saenger
Apr 21, 2010·Plasmid·Virginia S LioyJuan C Alonso
Oct 9, 2013·Acta Crystallographica. Section D, Biological Crystallography·Joëlle HuetAlexandre Wohlkönig
Jul 12, 2017·Frontiers in Microbiology·María Moreno-Del ÁlamoJuan C Alonso
Jan 13, 2019·Toxins·María Moreno-Del ÁlamoJuan C Alonso
Dec 19, 2020·Toxins·María Moreno-Del ÁlamoJuan C Alonso

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