Crystallization and preliminary X-ray diffraction analysis of a lectin from Canavalia maritima seeds

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Carlos Alberto de Almeida GadelhaBenildo S Cavada

Abstract

A lectin from Canavalia maritima seeds (ConM) was purified and submitted to crystallization experiments. The best crystals were obtained using the vapour-diffusion method at a constant temperature of 293 K and grew in 7 d. A complete structural data set was collected to 2.1 A resolution using a synchrotron-radiation source. The ConM crystal belongs to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 67.15, b = 70.90, c = 97.37 A. A molecular-replacement search found a solution with a correlation coefficient of 69.2% and an R factor of 42.5%. Crystallographic refinement is under way.

References

Jul 1, 1992·Immunological Investigations·M Barral-NettoB S Cavada
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Oct 1, 1995·Plant Physiology·W J Peumans, E J Van Damme
Jan 1, 1993·Agents and Actions·C A BentoC Barja-Fidalgo
Jun 8, 1999·Cellular Immunology·J L AndradeM Barral-Netto
Dec 11, 1999·Nucleic Acids Research·H M BermanP E Bourne
Dec 23, 1999·Current Opinion in Structural Biology·M Vijayan, N Chandra
Mar 7, 2000·Mediators of Inflammation·N M AlencarR A Ribeiro
Nov 28, 2000·Acta Crystallographica. Section D, Biological Crystallography·J BouckaertL Wyns
Aug 14, 2001·Memórias do Instituto Oswaldo Cruz·T BarbosaM Barral-Netto
Apr 8, 2003·Protein and Peptide Letters·Alexandre HavtHelena S A Monteiro
Jul 24, 2004·Acta Crystallographica. Section D, Biological Crystallography·Frederico B M B MorenoBenildo S Cavada

❮ Previous
Next ❯

Citations

Nov 2, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Frederico Bruno Mendes Batista MorenoWalter Filgueira de Azevedo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Acta Crystallographica. Section D, Biological Crystallography
Collaborative Computational Project, Number 4
Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
P DelatorreBenildo S Cavada
Journal of Structural Biology
Carlos Alberto de Almeida GadelhaBenildo S Cavada
© 2021 Meta ULC. All rights reserved