Crystallization and preliminary X-ray diffraction studies of a catechol-O-methyltransferase/inhibitor complex

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Maria L RodriguesMargarida Archer

Abstract

Inhibitors of the enzyme catechol-O-methyltransferase (COMT) are used as co-adjuvants in the therapy of Parkinson's disease. A recombinant form of the soluble cytosolic COMT from rat has been co-crystallized with a new potent inhibitor, BIA 8-176 [(3,4-dihydroxy-2-nitrophenyl)phenylmethanone], by the vapour-diffusion method using PEG 6K as precipitant. Crystals diffract to 1.6 A resolution on a synchrotron-radiation source and belong to the monoclinic space group P2(1), with unit-cell parameters a = 52.77, b = 79.63, c = 61.54 A, beta = 91.14 degrees.

References

Apr 1, 1991·DNA and Cell Biology·K LundströmI Ulmanen
Jul 1, 1971·Biochemical Pharmacology·E Blaschke, G Hertting
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Aug 16, 1995·Biochimica Et Biophysica Acta·K LundströmI Ulmanen
Aug 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T KarhunenP Panula
Mar 24, 1994·Nature·J VidgrenA Liljas
May 26, 2001·Acta Crystallographica. Section D, Biological Crystallography·M L RodriguesM A Carrondo
Sep 26, 2001·Protein Expression and Purification·M J BonifácioP Soares-da-Silva
Nov 1, 2003·Science·Ted M Dawson, Valina L Dawson
May 25, 2004·Science·J Timothy Greenamyre, Teresa G Hastings

❮ Previous
Next ❯

Citations

Jun 4, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Isabelle MottaFrederic Pincet
Aug 1, 2014·Journal of Medicinal Chemistry·László E Kiss, Patrício Soares-da-Silva

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.