Crystallization and preliminary X-ray diffraction analysis of a chitin-binding domain of hyperthermophilic chitinase from Pyrococcus furiosus

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Tsutomu NakamuraKoichi Uegaki

Abstract

The crystallization and preliminary X-ray diffraction analysis of the chitin-binding domain of chitinase from a hyperthermophilic archaeon, Pyrococcus furiosus, are reported. The recombinant protein was prepared using an Escherichia coli overexpression system and was crystallized by the hanging-drop vapour-diffusion method. An X-ray diffraction data set was collected to 1.70 A resolution. The crystal belonged to space group P4(3)2(1)2 or P4(1)2(1)2. The unit-cell parameters were determined to be a = b = 48.8, c = 85.0 A.

Citations

Oct 31, 2006·Extremophiles : Life Under Extreme Conditions·Hee-Jin KangKazuhiko Ishikawa
Aug 2, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Shouhei MineKoichi Uegaki
Dec 22, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Tsutomu NakamuraKoichi Uegaki
Jun 21, 2012·Protein Expression and Purification·Shouhei MineKoichi Uegaki
Mar 27, 2016·Extremophiles : Life Under Extreme Conditions·Konstantinos PapadimitriouConstantinos Vorgias
Feb 10, 2009·Bioscience, Biotechnology, and Biochemistry·Akihiro ItoNorikazu Ueyama

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