Crystallization and preliminary X-ray diffraction analysis of the electron-transfer complex between the terminal oxygenase component and ferredoxin in the Rieske non-haem iron oxygenase system carbazole 1,9a-dioxygenase

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Yuji AshikawaHideaki Nojiri

Abstract

Carbazole 1,9a-dioxygenase, which consists of an oxygenase component (CARDO-O) and the electron-transport components ferredoxin (CARDO-F) and ferredoxin reductase (CARDO-R), catalyzes dihydroxylation at the C1 and C9a positions of carbazole. The electron-transport complex between CARDO-O and CARDO-F crystallizes at 293 K using hanging-drop vapour diffusion with the precipitant PEG MME 2000 (type I crystals) or PEG 3350 (type II). Blossom-shaped crystals form from a pile of triangular plate-shaped crystals. The type I crystal diffracts to a maximum resolution of 1.90 A and belongs to space group P2(1), with unit-cell parameters a = 97.1, b = 89.8, c = 104.9 A, alpha = gamma = 90, beta = 103.8 degrees. Diffraction data for the type I crystal gave an overall Rmerge of 8.0% and a completeness of 100%. Its VM value is 2.63 A3 Da(-1), indicating a solvent content of 53.2%.

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Citations

Dec 5, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Kengo InoueHideaki Nojiri
Jun 8, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Yuji AshikawaHideaki Nojiri
Oct 3, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Kengo InoueHideaki Nojiri
Jul 9, 2008·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Takashi UmedaHideaki Nojiri
Nov 7, 2013·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Jun MatsuzawaHideaki Nojiri
Jul 2, 2021·Acta Crystallographica. Section D, Structural Biology·Yuji AshikawaHideaki Nojiri
May 26, 2009·Archives of Biochemistry and Biophysics·Michael TarasevDavid P Ballou

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