Crystallization and preliminary X-ray diffraction analysis of calexcitin from Loligo pealei: a neuronal protein implicated in learning and memory

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
G D E BeavenJ B Cooper

Abstract

The neuronal protein calexcitin from the long-finned squid Loligo pealei has been expressed in Escherichia coli and purified to homogeneity. Calexcitin is a 22 kDa calcium-binding protein that becomes up-regulated in invertebrates following Pavlovian conditioning and is likely to be involved in signal transduction events associated with learning and memory. Recombinant squid calexcitin has been crystallized using the hanging-drop vapour-diffusion technique in the orthorhombic space group P2(1)2(1)2(1). The unit-cell parameters of a = 46.6, b = 69.2, c = 134.8 A suggest that the crystals contain two monomers per asymmetric unit and have a solvent content of 49%. This crystal form diffracts X-rays to at least 1.8 A resolution and yields data of high quality using synchrotron radiation.

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Citations

Jan 12, 2010·Cases Journal·Vincent RodriguesAshutosh S Rao
Apr 7, 2016·Acta Crystallographica. Section F, Structural Biology Communications·Lucas ChataignerJonathan B Cooper
Mar 12, 2015·Acta Crystallographica. Section D, Biological Crystallography·P T ErskineJ B Cooper
Dec 7, 2021·Acta Crystallographica. Section F, Structural Biology Communications·Jingxu GuoJonathan B Cooper

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