Crystallization and preliminary X-ray diffraction analysis of human phosphate-binding protein

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Carlos Contreras-MartelEric Chabrière

Abstract

Human phosphate-binding protein (HPBP) was serendipitously discovered by crystallization and X-ray crystallography. HPBP belongs to a eukaryotic protein family named DING that is systematically absent from the genomic database. This apoprotein of 38 kDa copurifies with the HDL-associated apoprotein paraoxonase (PON1) and binds inorganic phosphate. HPBP is the first identified transporter capable of binding phosphate ions in human plasma. Thus, it may be regarded as a predictor of phosphate-related diseases such as atherosclerosis. In addition, HPBP may be a potential therapeutic protein for the treatment of such diseases. Here, the purification, detergent-exchange protocol and crystallization conditions that led to the discovery of HPBP are reported.

References

Jan 1, 1992·Annual Review of Cell Biology·C F Higgins
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Jun 25, 2002·The Journal of Biological Chemistry·Denis JossePatrick Masson
Jul 24, 2002·FEBS Letters·Anne BernaBruno Stuhlmüller
Dec 4, 2003·Acta Crystallographica. Section D, Biological Crystallography·A FokineE Chabriere

❮ Previous
Next ❯

Citations

Dec 21, 2010·Journal of Synchrotron Radiation·Mikael EliasEric Chabriere
Jul 19, 2011·Virology Journal·Thomas CherrierChristian Schwartz
Feb 18, 2010·PloS One·Jean-Marc CollombetEric Chabrière
Apr 6, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Frédérique RenaultDaniel Rochu
Sep 21, 2012·Acta Crystallographica. Section D, Biological Crystallography·Pankaj PanwarEva Pebay-Peyroula
Nov 15, 2008·Biological Chemistry·Antimo Di MaroMaria Rosaria Faraone-Mennella
Nov 23, 2007·Biochemical Society Transactions·D RochuP Masson
Mar 7, 2021·International Journal of Molecular Sciences·Elena PorzioGiuseppe Manco

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.