Crystallization and preliminary X-ray diffraction studies of two thermostable alpha-galactosidases from glycoside hydrolase family 36

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
M FoucaultP Gouet

Abstract

alpha-Galactosidases from thermophilic organisms have gained interest owing to their applications in the sugar industry. The alpha-galactosidases AgaA, AgaB and AgaA A355E mutant from Geobacillus stearothermophilus have been overexpressed in Escherichia coli. Crystals of AgaB and AgaA A355E have been obtained by the vapour-diffusion method and synchrotron data have been collected to 2.0 and 2.8 A resolution, respectively. Crystals of AgaB belong to space group I222 or I2(1)2(1)2(1), with unit-cell parameters a = 87.5, b = 113.3, c = 161.6 A. Crystals of AgaA A355E belong to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 150.1, c = 233.2 A.

References

Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Sep 15, 1995·Structure·G Davies, B Henrissat
Dec 1, 1995·Bioscience, Biotechnology, and Biochemistry·H ShibuyaI Kusakabe
Aug 15, 1996·European Journal of Biochemistry·E Margolles-ClarkM Penttilä
Apr 1, 1997·Bioscience, Biotechnology, and Biochemistry·H ShibuyaI Kusakabe
Feb 17, 1998·Enzyme and Microbial Technology·E LuonteriL Viikari
Nov 22, 2000·Carbohydrate Research·P SpangenbergR Mattes
Sep 22, 2001·Acta Crystallographica. Section D, Biological Crystallography·J Navaza
Dec 4, 2003·Acta Crystallographica. Section D, Biological Crystallography·Zui FujimotoHiroshi Mizuno
Mar 9, 2004·Journal of Molecular Biology·Scott C Garman, David N Garboczi
Apr 5, 2005·Acta Crystallographica. Section D, Biological Crystallography·Airlie J McCoyRandy J Read

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