Abstract
alpha-Galactosidases from thermophilic organisms have gained interest owing to their applications in the sugar industry. The alpha-galactosidases AgaA, AgaB and AgaA A355E mutant from Geobacillus stearothermophilus have been overexpressed in Escherichia coli. Crystals of AgaB and AgaA A355E have been obtained by the vapour-diffusion method and synchrotron data have been collected to 2.0 and 2.8 A resolution, respectively. Crystals of AgaB belong to space group I222 or I2(1)2(1)2(1), with unit-cell parameters a = 87.5, b = 113.3, c = 161.6 A. Crystals of AgaA A355E belong to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 150.1, c = 233.2 A.
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Mar 17, 2009·Applied Microbiology and Biotechnology·Yanan CaoBin Yao
Jul 1, 2010·Applied Biochemistry and Biotechnology·Sha LiJun Yao
Sep 27, 2012·The Journal of Biological Chemistry·Romain MerceronPatrice Gouet
May 30, 2019·Journal of Bacteriology·Kambiz Morabbi HeraviJosef Altenbuchner