Crystallization and preliminary X-ray diffraction analysis of CfaE, the adhesive subunit of the CFA/I fimbriae from human enterotoxigenic Escherichia coli

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Yong-Fu LiDi Xia

Abstract

Enterotoxigenic Escherichia coli (ETEC) represents a formidable food and waterborne diarrheal disease threat of global importance. The first step in ETEC pathogenesis is bacterial attachment to small-intestine epithelial cells via adhesive fimbriae, many of which are genetically related to the prototype colonization factor antigen I (CFA/I). The minor fimbrial subunit CfaE is required for initiation of CFA/I fimbrial assembly and mediates bacterial attachment to host cell-surface receptors. A donor-strand complemented variant of CfaE (dscCfaE) was expressed with a hexahistidine tag, purified to homogeneity and crystallized using the hanging-drop vapor-diffusion method. X-ray diffraction data sets were collected to 2.4 A resolution for both native and derivatized crystals and showed the symmetry of space group P6(2)22, with unit-cell parameters a = b = 142.9, c = 231.9 A. Initial phases were derived from the SIRAS approach and electron density showed two molecules in the crystallographic asymmetric unit. Sequence assignments were aided by anomalous signals from the selenium of an SeMet-derivatized crystal and from S atoms of a native crystal.

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Citations

Dec 14, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Zhiyong SuoRecep Avci
Jun 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yong-Fu LiEsther Bullitt
Mar 4, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Yong Fu LiDi Xia
Mar 15, 2014·Experimental & Molecular Medicine·David W PascualIrina Kochetkova
Aug 19, 2016·Zeitschrift für Kinder- und Jugendpsychiatrie und Psychotherapie·Viktoria Irlbauer-MüllerOliver Kratz

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