Crystallization and preliminary X-ray diffraction analysis of iron regulatory protein 1 in complex with ferritin IRE RNA

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Anna SeleznevaKarl Volz

Abstract

Iron regulatory protein 1 (IRP1) is a bifunctional protein with activity as an RNA-binding protein or as a cytoplasmic aconitase. Interconversion of IRP1 between these mutually exclusive states is central to cellular iron regulation and is accomplished through iron-responsive assembly and disassembly of a [4Fe-4S] cluster. When in its apo form, IRP1 binds to iron responsive elements (IREs) found in mRNAs encoding proteins of iron storage and transport and either prevents translation or degradation of the bound mRNA. Excess cellular iron stimulates the assembly of a [4Fe-4S] cluster in IRP1, inhibiting its IRE-binding ability and converting it to an aconitase. The three-dimensional structure of IRP1 in its different active forms will provide details of the interconversion process and clarify the selective recognition of mRNA, Fe-S sites and catalytic activity. To this end, the apo form of IRP1 bound to a ferritin IRE was crystallized. Crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 109.6, b = 80.9, c = 142.9 A, beta = 92.0 degrees. Native data sets have been collected from several crystals with resolution extending to 2.8 A and the structure has been solved by molecular replacement.

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Citations

Oct 15, 2009·Chemical Reviews·Elizabeth C Theil, Dixie J Goss
Sep 2, 2009·The Journal of Biological Chemistry·Mateen A KhanElizabeth C Theil
Apr 18, 2013·Gene·O'Neil Shand, Karl Volz
Jan 11, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Elizabeth C Theil
Jul 27, 2006·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Elizabeth C TheilXiaofeng Liu
Dec 28, 2010·Free Radical Biology & Medicine·Nikolai L Chepelev, William G Willmore
May 23, 2012·Biochimica Et Biophysica Acta·Cole P AndersonElizabeth A Leibold
Jun 29, 2013·Journal of Molecular Biology·Anna I SeleznevaKarl W Volz
Jul 20, 2006·Nature Chemical Biology·Tracey A Rouault
Jan 19, 2007·Journal of the American Society of Nephrology : JASN·Deliang ZhangTracey A Rouault
Aug 24, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Mateen A KhanJaved M Khan

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