Crystallization and preliminary X-ray diffraction analysis of salutaridine reductase from the opium poppy Papaver somniferum.

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Yasuhiro HigashiToni M Kutchan

Abstract

The opium poppy Papaver somniferum is the source of the narcotic analgesics morphine and codeine. Salutaridine reductase (SalR; EC 1.1.1.248) reduces the C-7 keto group of salutaridine to the C-7 (S)-hydroxyl group of salutaridinol in the biosynthetic pathway that leads to morphine in the opium poppy plant. P. somniferum SalR was overproduced in Escherichia coli and purified using cobalt-affinity and size-exclusion chromatography. Hexagonal crystals belonging to space group P6(4)22 or P6(2)22 were obtained using ammonium sulfate as precipitant and diffracted to a resolution of 1.9 A.

References

May 9, 1995·Biochemistry·H JörnvallD Ghosh
Mar 26, 1999·European Journal of Biochemistry·J N Tinguely, B Wermuth
Apr 27, 2002·The Journal of Biological Chemistry·Charlotta FillingUdo Oppermann
Sep 17, 2002·European Journal of Biochemistry·Yvonne KallbergBengt Persson
Aug 22, 2003·Protein Science : a Publication of the Protein Society·Katherine A Kantardjieff, Bernhard Rupp
Apr 1, 1965·Journal of the Chemical Society·D H BARTONH RAMUZ
Apr 1, 2005·PLoS Biology·Masahiro TanakaCynthia L Adams
Oct 4, 2007·Organic & Biomolecular Chemistry·Raynard BatemanKevan M Shokat
Oct 2, 2008·The Journal of Biological Chemistry·Raynard L BatemanKevan M Shokat
Nov 26, 2008·Chemico-biological Interactions·Bengt PerssonUdo Oppermann
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

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Citations

Dec 21, 2010·The Journal of Biological Chemistry·Yasuhiro HigashiThomas J Smith
Sep 17, 2013·Physiologia Plantarum·Nidarshana ChaturvediAjit K Shasany
Apr 2, 2015·Critical Reviews in Analytical Chemistry·Andrei A BunaciuHassan Y Aboul-Enein
Sep 2, 2016·Journal of Industrial Microbiology & Biotechnology·Miguel Suástegui, Zengyi Shao

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