Crystallization and preliminary X-ray diffraction analysis of boar seminal plasma spermadhesin PSP-I/PSP-II, a heterodimer of two CUB domains

FEBS Letters
A RomeroJ J Calvete

Abstract

Boar spermadhesin PSP-I/PSP-II (M(r) 29 000-30 000), a non-covalent heterodimer of two CUB domains, was crystallized in two crystal forms. Complete diffraction data sets for hexagonal (space group P6(1,5)22) and trigonal (space group P3(1,2)21) crystals have been collected up to 2.9 and 2.5 angstrom resolution, respectively. Cell constants of the hexagonal and trigonal crystal forms are a=b=87.2 angstrom, c=152.4 angstrom, and a=b=96.2 angstrom, c=70.8 angstrom, respectively. The calculated packing parameters (Vm) are 2.8 and 3.2 angstrom(3)/DA for the hexagonal and trigonal crystal forms, respectively, indicating that, in both cases, the asymmetric unit is constituted by one PSP-I/PSP-II heterodimer. This paper reports the first crystals of a protein built up by a CUB domain architecture.

References

Feb 28, 1992·Biochemical and Biophysical Research Communications·F WempeK H Scheit
Jun 1, 1992·Archives of Biochemistry and Biophysics·K J RutherfurdS C Kwok
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
May 15, 1995·European Journal of Biochemistry·Z DostálováE Töpfer-Petersen
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T KeitelU Heinemann
Feb 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·D I LiaoO Herzberg
Jan 27, 1994·Nature·J EmsleyS P Wood
Dec 1, 1993·European Journal of Biochemistry·J J CalveteE Töpfer-Petersen
May 20, 1993·Journal of Molecular Biology·P Bork, G Beckmann
Feb 5, 1996·FEBS Letters·J J CalveteE Töpfer-Petersen

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Citations

Jun 1, 2005·Gene·Bianca HaaseTosso Leeb
Jun 1, 1997·Cytokine & Growth Factor Reviews·H G Wisniewski, J Vilcek
Oct 23, 1997·Nature Structural Biology·A RomeroJ J Calvete
May 1, 2012·Reproduction, Fertility, and Development·Antônia Sâmia F NascimentoBenildo S Cavada
Feb 1, 1997·European Journal of Biochemistry·H J Gabius
Jan 5, 2002·Molecular Reproduction and Development·Mahnaz Ekhlasi-HundrieserEdda Töpfer-Petersen
Mar 28, 2006·Current Topics in Developmental Biology·Julian L Wong, Gary M Wessel
Jun 1, 2007·Molecular Reproduction and Development·Luciana Magalhães MeloGandhi Rádis-Baptista
Sep 18, 1998·Andrologia·E Töpfer-PetersenJ J Calvete
Mar 11, 2021·Theriogenology·Luan Sitó da SilvaFlavio Augusto Vicente Seixas

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