Crystallization and preliminary X-ray studies on the reaction center-light-harvesting 1 core complex from Rhodopseudomonas viridis

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Shinya SaijoT Odahara

Abstract

The reaction center-light-harvesting 1 (RC-LH1) core complex is the photosynthetic apparatus in the membrane of the purple photosynthetic bacterium Rhodopseudomonas viridis. The RC is surrounded by an LH1 complex that is constituted of oligomers of three types of apoproteins (alpha, beta and gamma chains) with associated bacteriochlorophyll bs and carotenoid. It has been crystallized by the sitting-drop vapour-diffusion method. A promising crystal diffracted to beyond 8.0 A resolution. It belonged to space group P1, with unit-cell parameters a = 141.4, b = 136.9, c = 185.3 A, alpha = 104.6, beta = 94.0, gamma = 110.7 degrees. A Patterson function calculated using data between 15.0 and 8.0 A resolution suggested that the LH1 complex is distributed with quasi-16-fold rotational symmetry around the RC.

References

Nov 1, 1988·Quarterly Reviews of Biophysics·W Kühlbrandt
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Oct 26, 1999·Acta Crystallographica. Section D, Biological Crystallography·J W Pflugrath
Feb 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Simon ScheuringJean-Louis Rigaud
Dec 13, 2003·Science·Aleksander W RoszakRichard J Cogdell

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