Crystallization and preliminary X-ray characterization of a lectin from Cicer arietinum (chickpea)

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Uma V KatreC G Suresh

Abstract

The lectin isolated from mature seeds of Cicer arietinum (CAL) agglutinates pronase-treated rabbit and human erythrocytes and its haemagglutination activity is inhibited by fetuin and desialated fetuin but not by simple monosaccharides or oligosaccharides. The purified lectin is a dimer of molecular weight 43,000 Da composed of two identical subunits (MW 21,500), as confirmed by SDS-PAGE. The lectin has been crystallized using the hanging-drop vapour-diffusion method at 295 K over a well solution containing 0.2 M sodium acetate, 0.1 M sodium phosphate buffer pH 6.5 and 14%(w/v) polyethylene glycol 8000. The triangular prism-shaped crystals belong to space group R3 and have unit-cell parameters a = b = 81.2, c = 69.4 A. The diffraction data are 93.8% complete to 2.3 A Bragg spacing with an Rmerge of 0.103.

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Citations

Jan 21, 2016·Journal of Evolutionary Biology·Uroš SavkovićBiljana Stojković
Mar 15, 2013·International Journal of Biological Macromolecules·Madhurima S WakankarSushama M Gaikwad
Aug 9, 2011·Journal of Agricultural and Food Chemistry·Alessio ScarafoniMarcello Duranti
Dec 8, 2007·International Journal of Biological Macromolecules·Uma V KatreSushama M Gaikwad
Mar 29, 2019·Protein and Peptide Letters·Sameer Suresh BhagyawantNidhi Srivastava
Apr 26, 2018·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Ajay Kumar GautamSameer S Bhagyawant

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