Crystallization and preliminary X-ray crystallographic study of the wild type and two mutants of the CP1 hydrolytic domain from Aquifex aeolicus leucyl-tRNA synthetase

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Vincent CuraJean Cavarelli

Abstract

The editing or hydrolytic CP1 domain of leucyl-tRNA synthetase (LeuRS) hydrolyses several misactivated amino acids. The CP1 domain of Aquifex aeolicus LeuRS was expressed, purified and crystallized by the hanging-drop vapour-diffusion method using ammonium sulfate as precipitant. Crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 38.8, b = 98.4, c = 116.7 A. Crystals diffract to beyond 1.8 A resolution and contain two monomers in the asymmetric unit. Two CP1 mutants in which a conserved threonine residue essential for the fidelity of the hydrolytic pathway is mutated to alanine or glutamic acid have also been expressed and crystallized. Crystals of the two CP1 mutants are isomorphs of the wild type and diffract to beyond 1.9 A resolution. All structures were solved by molecular-replacement techniques.

References

Sep 24, 1999·Biochimie·S A MartinisC Florentz
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·P J Beuning, K Musier-Forsyth
Jun 20, 2002·Journal of the American Chemical Society·Richard S Mursinna, Susan A Martinis
Aug 28, 2002·The Journal of Biological Chemistry·Min-Gang XuEn-Duo Wang
Apr 30, 2003·Molecular Cell·Tommie L LincecumStephen Cusack
Sep 2, 2003·The Journal of Biological Chemistry·Kirk BeebePaul Schimmel
Dec 16, 2003·The Journal of Biological Chemistry·Ryuya FukunagaShigeyuki Yokoyama

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Citations

Sep 20, 2011·The Journal of Physical Chemistry. B·Mauro Boero

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