Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Kyung-Hee RheeKwang Yeon Hwang

Abstract

Fatty acid-CoA racemase plays an important role in the beta-oxidation of branched-chain fatty acids and fatty-acid derivatives as it catalyzes the conversion of several (2R)-branched-chain fatty acid-CoAs to their (2S)-stereoisomers. Fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv has been purified to homogeneity and crystallized by the hanging-drop vapour-diffusion method with polyethylene glycol 4000 as precipitant. The crystals belong to the trigonal space group P3(1) or P3(2), with unit-cell parameters a = b = 109.56, c = 147.97 A. The asymmetric unit contains six monomers, corresponding to a VM value of 2.15 A3 Da(-1). A complete native data set has been collected at 2.7 A resolution using a synchrotron-radiation source.

References

Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Sep 1, 1995·Journal of Neuropathology and Experimental Neurology·R J WandersP G Barth
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Jan 30, 2003·Acta Crystallographica. Section D, Biological Crystallography·Prasenjit BhaumikRik K Wierenga
Mar 14, 2003·Biochimica Et Biophysica Acta·Ronald J A WandersMatthew D Lloyd
Oct 7, 2004·Molecular Genetics and Metabolism·R J A Wanders

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Citations

Feb 5, 2013·Progress in Lipid Research·Matthew D LloydTimothy J Woodman
May 15, 2009·Cellular Microbiology·Sabine Ehrt, Dirk Schnappinger

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