PMID: 1203253Dec 18, 1975Paper

Crystallization and properties of rat liver malate dehydrogenase (decarboxylating) (NADP)

Biochimica Et Biophysica Acta
F WadaY Sakamoto

Abstract

Rat liver malate dehydrogenase (decarboxylating) (NADP) ((L-malate: NADP) oxidoreductase (oxaloacetate-decarboxylating), EC 1.1.1.40) was purified and crystallized from medium containing 30 mM Tris-HCl buffer (pH 7.7), 5 mM MgCl2 and 2 mM 2-mercaptoethanol. The enzyme formed rhomboid crystals free from coenzyme, and appeared homogeneous on isoelectric focusing. The crystalline enzyme had an isoelectric point of pH 6.3. Amino acid analysis showed that it contained more acidic amino acids than basic ones.

References

Oct 1, 1971·Biochimica Et Biophysica Acta·F IsoashiF Wada
Oct 1, 1974·The Biochemical Journal·G Murphy, D G Walker
Jan 1, 1971·Journal of Biochemistry·T SaitoK Tomita
Apr 27, 1971·Biochimica Et Biophysica Acta·P Righetti, J W Drysdale

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Citations

Jan 1, 1981·The International Journal of Biochemistry·J SwierczyńskiM Zydowo
Jan 1, 1981·The International Journal of Biochemistry·D M Kirschenbaum
Jan 1, 1988·The International Journal of Biochemistry·F Taroni, S Di Donato
Jan 1, 1977·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·E F SkorkowskiZ Aleksandrowicz
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·E MaderoA Garrido-Pertierra
Jan 1, 1995·The International Journal of Biochemistry & Cell Biology·G BukatoJ Swierczyński
Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·F A MomanyA R Larrabee
Oct 15, 1991·European Journal of Biochemistry·M Zelewski, J Swierczyński
Jan 1, 1988·Molecular and Cellular Endocrinology·K M McHugh, R L Drake
Apr 1, 1982·Analytical Biochemistry·J T Chang, G G Chang

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