Crystallization and X-ray analysis of 2-deoxy-scyllo-inosose synthase, the key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Eriko NangoTadashi Eguchi

Abstract

A recombinant 2-deoxy-scyllo-inosose synthase from Bacillus circulans has been crystallized at 277 K using PEG 4000 as precipitant. The diffraction pattern of the crystal extends to 2.30 A resolution at 100 K using synchrotron radiation at the Photon Factory. The crystals are monoclinic and belong to space group P2(1), with unit-cell parameters a = 80.5, b = 70.4, c = 83.0 A, beta = 117.8 degrees. The presence of two molecules per asymmetric unit gives a crystal volume per protein weight (VM) of 2.89 A3 Da(-1) and a solvent constant of 57.4% by volume.

Citations

May 13, 2010·Bioscience, Biotechnology, and Biochemistry·Hideyuki TamegaiChiaki Kato
Mar 29, 2007·Natural Product Reports·Patricia M Flatt, Taifo Mahmud
Nov 23, 2006·Natural Product Reports·Nicholas M Llewellyn, Jonathan B Spencer
Dec 30, 2006·Chembiochem : a European Journal of Chemical Biology·Xiumei WuTaifo Mahmud

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