Crystallization of a truncated soluble human semicarbazide-sensitive amine oxidase

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Emma JakobssonGerard J Kleywegt

Abstract

Human semicarbazide-sensitive amine oxidase (SSAO) is a homodimeric copper-containing monoamine oxidase that occurs in both a membrane-bound and a soluble form. SSAO is also known as vascular adhesion protein-1 (VAP-1). A truncated soluble form of human SSAO (comprising residues 29-763) was expressed in human embryonic kidney 293 cells and purified to homogeneity. Tetragonal crystals were obtained and a data set extending to 2.5 A was collected. The crystals are merohedrally twinned and the estimation of the twinning fraction was complicated by pseudo-symmetry and the anisotropic character of the crystals. Using a recently developed method for twinning detection that is insensitive to phenomena such as anisotropy or pseudo-symmetry [Padilla & Yeates (2003), Acta Cryst. D59, 1124-1130], the twinning fraction was estimated to be 0.3. The structure was eventually solved by molecular replacement in space group P4(3).

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Citations

Jan 19, 2013·Journal of Neural Transmission·Eva Bligt-LindénTiina Annamaria Salminen
Aug 23, 2011·Amino Acids·Bonaiuto EmanuelaMaria Luisa Di Paolo
Jan 11, 2014·Archives of Biochemistry and Biophysics·Joel FinneyMinae Mure
Nov 2, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·David B LangleyJ Mitchell Guss
Dec 5, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Anthony P DuffJ Mitchell Guss
Jul 10, 2009·Cellular and Molecular Life Sciences : CMLS·Sam KaitaniemiKati Elima
Jun 17, 2011·Expert Opinion on Therapeutic Patents·Petra DunkelPéter Mátyus

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